ISSN:
1432-0428
Keywords:
B-cell microtubules
;
spontaneous diabetes
;
spiny mice (Acomys cahirinus)
;
DBM mice
;
isolated islets
;
vincristine-induced crystals
;
electron microscopy
;
morphometry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The pancreatic B-cell contains microtubules, which are thought to participate in the process of insulin release. In order to disclose a possible abnormality of this B-cell microtubular system in animals with islet dysfunction, isolated islets from normal rats and mice, as well as from diabetic mutant mice (DBM mice) and from spiny mice (Acomys cahirinus) were incubated in the presence of vincristine, which causes the precipitation of the microtubular protein into paracrystalline deposits. Ultrastructural examination of the islets indicated that the volume-density of vincristine-induced deposits was markedly reduced in B-cells of spiny mice, when compared to that found in normal rats and mice and DBM mice. Exposure of the islets from spiny mice to a high glucose concentration, concomittantly to vincristine, caused a further reduction in vincristine-induced crystals content of the B-cell. It is speculated that an impairment of the B-cell microtubular system may account for the deficiency of insulin release found in spiny mice.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00422821
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