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  • 1
    Electronic Resource
    Electronic Resource
    In vitro systems for investigating group B streptococcal: host cell and extracellular matrix interactions (1998)
    Springer
    Methods in cell science 20 (1998), S. 191-201 
    Details
    ISSN: 1573-0603
    Keywords: Adherence ; Chorioamnion ; Extracellular matrix ; Group B. streptococci ; Invasion fibronectin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Streptococcus agalactiae or group B streptococci (GBS) are gram-positive diplococci and are the leading bacterial cause of pneumoniae, sepsis, and meningitis in neonates. Neonatal GBS infections may occur prior to or during birth. GBS have been cultured from the chorioamnionic membrane of pregnant women and have therefore been associated with chorioamnionitis and premature labor. A potential route for GBS to establish infection of a neonate would be to penetrate the placental membrane of colonized pregnant women. In our laboratory, we have constructed in vitro systems to emulate certain events during the colonization and invasion of host epithelial cell tissues by GBS. By utilizing techniques to grow primary cultures of both chorion cells and amnion cells isolated from human C-section placentas, we have established a relevant model to investigate certain aspects of GBS adherence and invasion into the placental membrane. To identify relevant molecules required for GBS to colonize the multiple tissues it encounters during an infection, we have applied a variety of biochemical approaches with host cell membrane preparations as well as purified extracellular matrix proteins. These techniques are enabling us to further characterize the pathogenic mechanisms utilized by GBS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009866222748
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A simple microtiter plate screening assay for bacterial invasion or adherence (1998)
    Springer
    Methods in cell science 20 (1998), S. 107-111 
    Details
    ISSN: 1573-0603
    Keywords: Bacterial adherence ; Bacterial invasion ; Bacteriological techniques ; Invasion assay ; Microbial colony count ; Soft agar
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Many bacteriologic studies, including cellular invasion and adherence assays, require enumeration of viable organisms or colony forming units. When attempting to screen large numbers of clinical or environmental isolates or laboratory-derived mutants for differences in invasion or adherence phenotype, standard plating methods can be cumbersome and severely limit the number of organisms or conditions which can be tested. As a potential alternative, we describe a simple, rapid and inexpensive soft agar-based technique for semi-quantitative determination of bacterial colony counts directly within the wells of a 96-well microtiter plate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009830606819
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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