ISSN:
1059-910X
Keywords:
Pre-embedding method
;
Post-embedding method
;
Non-embedding method
;
Protein A-gold technique
;
Double labeling
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Natural Sciences in General
Notes:
Recent advances in ultrastructural immunohistochemistry have provided insight into not only the subcellular localization of single antigens but also the colocalization of two distinct antigens in the same cellular constituent. In the field of pituitary pathology, precise identification of cell types, mechanism of processing, and dynamic intracellular transportation of hormones, as well as produciton of multiple hormones in the same cells of nontumorous and neoplastic adenohypophyses, have been documented by use of these techniques. The present review deals with the use of major methods for ultrastructural immunohistochemistry including pre-, post-, and non-embedding methods, paricularly focusing on their application to human pituitary pathology. Problems of tissue processing and a protocol for double labeling technique using the protein A-gold complex are also described.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jemt.1070200204
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