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  • 1
    Electronic Resource
    Electronic Resource
    Genetic analysis of the obligate parasitic barley powdery mildew fungus based on RFLP and virulence loci (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 705-712 
    Details
    ISSN: 1432-2242
    Keywords: Erysiphe graminis ; Genetic map ; Repetitive ; DNA ; RFLP ; Virulence genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genome organization of the biotrophic barley powdery mildew fungus was studied using restriction fragment length polymorphism (RFLP). Genomic DNA clones containing either low-or multiple-copy sequences appeared to be the best RFLP markers, as they frequently revealed polymorphisms that could be readily detected. A total of 31 loci were identified using 11 genomic DNA clones as probes. Linkage analysis of the 31 RFLP loci and five virulence loci resulted in the construction of seven groups of linked loci. Two of these contained both RFLP markers and virulence genes. RFLP markers were found to be very efficient in characterizing mildew isolates, as only three markers were necessary to differentiate 28 isolates. The DNA of the barley powdery mildew fungus appeared to contain a considerable number of repetitive sequences dispersed throughout the genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226887
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Localization of the Laevigatum powdery mildew resistance gene to barley chromosome 2 by the use of RFLP markers (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 897-900 
    Details
    ISSN: 1432-2242
    Keywords: Barley leaf stripe ; Erysiphe graminis f.sp hordei ; Heterodera avena ; Linkage map ; Ml(La)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The powdery mildew disease resistance gene Ml(La) was found to belong to a locus on barely chromosome 2. We suggest that this locus be designated MlLa. Linkage analysis was carried out on 72 chromosome-doubled, spring-type progeny lines from a cross between the winter var ‘Vogelsanger Gold’ and the spring var ‘Alf’. A map of chromosome 2 spanning 119cM and flanked by two peroxidase gene loci was constructed. In addition to the Laevigatum resistance locus the map includes nine RFLP markers, the two peroxidase gene loci and the six-row locus in barley.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225035
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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