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  • 1
    Publication Date: 2020-05-13
    Language: English
    Type: conferenceobject , doc-type:conferenceObject
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  • 2
    ISSN: 0378-1119
    Keywords: High molecular weight DNA ; RAPD ; RFLP ; contig ; fingerprint ; restriction fragment length polymorphism ; stability
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Plant pathology 45 (1996), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Genetic variation of the barley powdery mildew fungus (Erysiphe graminis f.sp. hordei) was estimated in three Danish local populations. Genetic variation was estimated from the variation amongst clones of Egh, and was therefore an estimate of the maximum genetic variation in the local populations. The average gene diversity, Ĥ, was estimated as 0.84. The effective population size was estimated as: log10 (N^e) = 0.64 − log10(μ), or 4.4 × 109, assuming a nucleotide mutation rate ( μ) of 10−9 per base per generation. There was no significant genetic differentiation between locations.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 121 (2002), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Three quantitative trait loci (QTL) for scald resistance in barley were identified and mapped in relation to molecular markers using a population of chromosome doubled-haploid lines produced from the F1 generation of a cross between the spring barley varieties ‘Alexis’ and ‘Regatta’. Two field experiments were conducted in Denmark and two in Norway to assess disease resistance. The percentage leaf area covered with scald (Rhynchosporium secalis) ranged from 0 to 40% in the 189 doubled-haploid (DH) lines analysed. One quantitative trait locus was localized in the centromeric region of chromosome 3H, Qryn3, using the MAPQTL program. MAPQTL was unable to provide proper localization of the other two resistance genes and so a non-interval QTL mapping method was used. One was found to be located distally to markers on chromosome 4H (Qryn4) and the other, Qryn6, was located distally to markers on chromosome 6H. The effects of differences between the Qryn3, Qryn4 and Qryn6 alleles in two barley genotypes for the QTL were estimated to be 8.8%, 7.3% and 7.0%, respectively, of leaf covered by scald. No interactions between the QTLs were found.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of virology 14 (1964), S. 657-673 
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Electron microscopical studies based on negative staining, shadow casting, thin-sectioning, and enzymatic analysis revealed fine structures typical of pox viruses in virions of contagious pustular dermatitis of sheep and bovine papular stomatitis. There are distinct differences from vaccinia virus. The surrounding membrane and the filaments are thicker. The double element, the peripheral protein layer and the inner body with the triplet of tube like strands are smaller, corresponding to the reduced dimensions of the virions. With regard to anomalous forms of virions, reaction upon enzymatic proteolysis and developmental stages no differences were found. Shadow casting cleared up, that the filaments do not cross really but that the criss-cross pattern in negative stained specimens is induced by superimposition of top and bottom images. It is proposed to classify both viruses together with the virus of milker's nodules in a pox subgroup called “paravaccinia viruses”.
    Notes: Zusammenfassung Die elektronenmikroskopische Untersuchung der Erreger des Ecthyma contagiosum der Schafe und der Stomatitis papulosa der Rinder mit den Methoden der Negativkontrastierung, der Bedampfung, der Ultrahistologie und der enzymatischen Analyse führte zur Darstellung der vom Vaccinevirus bekannten typischen Strukturen der Pockenviren mit verschiedenen Abweichungen im einzelnen. Die umhüllende Membran und die Filamente sind dicker, das Doppelelement, die periphere Proteinschicht und der Innenkörper mit dem Triplett röhrenförmiger Stränge sind den geringen Abmessungen der Virionen entsprechend kleiner als beim Vaccinevirus. Bezüglich anomaler Formen der Virionen, des Verhaltens gegenüber proteolytischen Enzymen und der Entwicklungsstadien ergab sich kein Unterschied. Die bedampften Präparate zeigten, daß sich die Filamente der Membran nicht kreuzen, sondern daß sich die entsprechenden Bilder nach Negativkontrastierung durch Projektion von Ober- und Unterseite ergeben. Es wird vorgeschlagen, in der Pockengruppe Orf- und BPS-sowie das Melkerknotenvirus zur Untergruppe der „Paravaccineviren“ zusammenzufassen.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 79 (1990), S. 705-712 
    ISSN: 1432-2242
    Keywords: Erysiphe graminis ; Genetic map ; Repetitive ; DNA ; RFLP ; Virulence genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genome organization of the biotrophic barley powdery mildew fungus was studied using restriction fragment length polymorphism (RFLP). Genomic DNA clones containing either low-or multiple-copy sequences appeared to be the best RFLP markers, as they frequently revealed polymorphisms that could be readily detected. A total of 31 loci were identified using 11 genomic DNA clones as probes. Linkage analysis of the 31 RFLP loci and five virulence loci resulted in the construction of seven groups of linked loci. Two of these contained both RFLP markers and virulence genes. RFLP markers were found to be very efficient in characterizing mildew isolates, as only three markers were necessary to differentiate 28 isolates. The DNA of the barley powdery mildew fungus appeared to contain a considerable number of repetitive sequences dispersed throughout the genome.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 89 (1994), S. 676-681 
    ISSN: 1432-2242
    Keywords: Hordeum vulgare ssp ; spontaneum Hordeum vulgare ssp ; vulgare ; Genetic distance ; Fixation index ; Restriction fragment length polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetic variability of cultivated and wild barley, Hordeum vulgare ssp. vulgare and spontaneum, respectively, was assessed by RFLP analysis. The material consisted of 13 European varietes, single-plant offspring lines of eight land races from Ethiopia and Nepal, and five accessions of ssp. spontaneum from Israel, Iran and Turkey. Seventeen out of twenty-one studied cDNA and gDNA probes distributed across all seven barley chromosomes revealed polymorphism when DNA was digested with one of four restriction enzymes. A tree based on genetic distances using frequencies of RFLP banding patterns was estimated and the barley lines clustered into five groups reflecting geographical origin. The geographical groups of land-race lines showed less intragroup variation than the geographical groups of spontaneum lines. The group of European varieties, representing large variation in agronomic traits, showed an intermediate level. The proportion of gene diversity residing among geographical groups (FST) varied from 0.19 to 0.94 (average 0.54) per RFLP pattern, indicating large diversification between geographical groups.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 79 (1990), S. 56-64 
    ISSN: 1432-2242
    Keywords: Barley powdery mildew ; Gene-for-gene hypothesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The obligate parasitic fungus, Erysiphe graminis f. sp. hordei, was found to harbour plasmid-like extrachromosomal DNA. A 1.35-kb fragment of this 9kb plasmid was cloned into the pUC12 vector. No homology was detected to nuclear or mitochondrial DNA. As only about half of the 27 isolates examined contained plasmid-like DNA, this appears to be inessential for fungal survival. The plasmid is frequent in European isolates and is found in both newly collected isolates and in isolates kept under laboratory conditions for many years. No correlation between presence of plasmid and specific avirulence/virulence genes was found. The plasmid appear to be located in the mitochondria.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-2242
    Keywords: Barley leaf stripe ; Erysiphe graminis f.sp hordei ; Heterodera avena ; Linkage map ; Ml(La)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The powdery mildew disease resistance gene Ml(La) was found to belong to a locus on barely chromosome 2. We suggest that this locus be designated MlLa. Linkage analysis was carried out on 72 chromosome-doubled, spring-type progeny lines from a cross between the winter var ‘Vogelsanger Gold’ and the spring var ‘Alf’. A map of chromosome 2 spanning 119cM and flanked by two peroxidase gene loci was constructed. In addition to the Laevigatum resistance locus the map includes nine RFLP markers, the two peroxidase gene loci and the six-row locus in barley.
    Type of Medium: Electronic Resource
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