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  • Flow cytometry  (2)
  • Benzoate  (1)
  • Carbamyl derivatives  (1)
  • Chironomidae  (1)
  • 1
    ISSN: 1432-0584
    Keywords: Reticulocyte ; Flow cytometry ; Bone marrow transplantation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Several flow-cytometric methods for reticulocyte enumeration in whole blood have been developed, with different degrees of practical use. Recently, a new fluorochrome, 1′,3′-diethyl-4,2′-quinolylthyacianine (DEQTC) was proposed in a brief report, as an alternative to thiazole orange for reticulocyte counting. We have evaluated the usefulness of this fluorescent stain by assessing the optimal conditions for the flow-cytometric analysis, and by comparing in double-blind assays the quantitative results of this technique with those obtained by manual counting with brilliant cresyl blue. Our results show that flow cytometry with DEQTC is highly correlated to the manual method (r=0.95–0.99), supporting the interest of this particular stain and of flow cytometry for routine laboratory work in hematology.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European journal of pediatrics 148 (1989), S. 540-542 
    ISSN: 1432-1076
    Keywords: Sodium benzoate ; Hyperammonaemia ; Carbamyl derivatives
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Sodium benzoate has been recommended for the treatment of hyperammonaemia in humans. However, benzoate potentiates ammonia toxicity and reduces urea synthesis in vitro and in vivo by decreasing the intramitochondrial levels of N-acetyl glutamate. Pretreatment of mice with carbamyl glutamate, a structural analogue of N-acetyl glutamate, decreases mortality induced by ammonium acetate and sodium benzoate administration. The protective effect of carbamyl glutamate is accompanied by an increase in urea production and of carbamyl phosphate synthetase activity.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    European journal of pediatrics 138 (1982), S. 186-187 
    ISSN: 1432-1076
    Keywords: Benzoate ; Ammonia toxicity ; Hyperammonemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effect of sodium benzoate on mice acutely intoxicated with ammonium acetate has been investigated. Benzoate potentiates ammonia toxicity and increases the blood and brain levels of ammonia in mice. The implications of these findings to the treatment with benzoate of hyperammonemia in man are discussed.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-203X
    Keywords: Key words Cryopreservation ; Flow cytometry ; Freezing stress ; Oryza sativa L. ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The progenies of rice plants (Oryza sativa L.) differentiated from calli that had been cryopreserved and from control (non-cryopreserved) calli were used to study the influence of selection pressure during cryopreservation. The phenotypic evaluation of these progenies was based mainly on the response of seedlings and calli to freezing stress and on the characterization of protoplast and cell populations by flow cytometric analyses. The patterns of response to freezing stress, as well as the variations in some morphological and physiological cell parameters, were unrelated to the origin (cryopreserved or control calli) of the parental plants.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5125
    Keywords: Chironomidae ; Trichoptera ; distribution ; glacial relict ; Ireland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Killarney region, south-west Ireland, has a mild oceanic climate, yet elements of the flora and fauna in the area appear to have disjunct distributions. Eleven chironomid and two trichopteran taxa, which are considered cold stenothermous and generally occur outside Ireland at high altitudes or northern latitudes, are found in waters around 25 metres above sea level in Killarney. These are believed to be glacial relict elements which have survived in the region to live with a fauna depauperate in comparison to western Europe.
    Type of Medium: Electronic Resource
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