ISSN:
1432-0428
Keywords:
Class II MHC antigen
;
I-A antigen
;
NOD mouse
;
Type 1 diabetes
;
insulitis
;
pancreatic B cell
;
autoimmunity
;
pathogenesis
;
pancreatic islet
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary To elucidate the role of class II major histocompatibility complex antigen expression on pancreatic B cells in the development of diabetes in the non-obese diabetic (NOD) mouse, indirect immunofluorescence was employed for I-A staining on Bouin-fixed pancreas sections of NOD mice (I-A of which was reported as d), B10.GD (I-A, d), BALB/c (I-A, d) and C3H/He (I-A, k). I-A positive islets were observed in all NOD mice examined. Positive reaction was detected in islets both with and without lymphocytic infiltrations. Double staining with anti-insulin, glucagon, somatostatin or pan creatic polypeptide antibodies revealed that I-A positive cells corresponded with insulin cells, while other types of pancreatic islet cells were virtually negative for I-A. Weaker staining was seen in islets of B10.GD and, to a lesser extent, in those of BALB/c mice. C3H/He mouse islet cells showed no I-A expression. These results demonstrated the expression of I-A antigens on pancreatic B cells in the NOD mouse.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00274580
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