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  • In situ hybridisation  (4)
  • Biochemistry and Biotechnology  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 65 (1983), S. 145-147 
    ISSN: 1432-2242
    Keywords: Diploid wheat ; Nucleolus organiser ; In situ hybridisation ; Telocentric markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The two nucleolus organiser chromosomes of diploid wheat are identified as 1A and 5A by the combination of in situ hybridisation and cytological markers.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 58 (1980), S. 97-100 
    ISSN: 1432-2242
    Keywords: Nucleolus organizers ; In situ hybridisation ; Diploid wheats ; Ribosomal RNA genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Labelled RNA, transcribed in vitro from wheat ribosomal DNA cloned in a bacterial plasmid, has been hybridised to metaphase chromosomes of five diploid wheats. Autoradiography of the chromosomes has provided unequivocal evidence that these genotypes possess two pairs of nucleolus organizer chromosomes. The diploid wheat accessions used possess widely differing numbers of ribosomal RNA genes.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Triticum timopheevi ; Chromosomes ; C-banding ; In situ hybridisation ; Heterochromatin ; Translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The chromosomes of the tetraploid wheats Triticum timopheevi (Genome AAGG) and T. araraticum (Genome AAGG) were C-banded at mitosis. The identity of the banded and unbanded chromosomes was then established by firstly making comparisons with the hexaploid species T. zhukovskyi which has the genome formula AAAAGG. Secondly, the meiotic pairing in F1 hybrids between T. timopheevi and diploid wheats was examined by means of C-banding. The results showed that the banded chromosomes belonged to the G genome, while the unbanded chromosomes belonged to the A genome. Only one of the two pairs of satellited chromosomes had strong heterochromatic bands. The relationship between the genomes of T. timopheevi and T. dicoccum (Genome AABB) was then assessed at meiosis in hybrids between these species, using the techniques of C-banding and in situ hybridisation of a cloned ribosomal RNA gene probe. It was concluded that there were differences both in the amount and distribution of heterochromatin and also translocation differences between the species.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 61 (1982), S. 285-288 
    ISSN: 1432-2242
    Keywords: Nucleolar organisers ; In situ hybridisation ; Tetraploid and hexaploid wheats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The technique of in situ hybridisation of cloned ribosomal DNA has been used to establish the numbers of nucleolar organising sites in a range of tetraploid and hexaploid wheats.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 15 (1973), S. 1075-1080 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The isolation and structure determination of a new microbial product, (+)- (123/45)-2,3,4,5-tetrahydroxy-1-cyclohexanemethanol is described. This product was detected in the fermentation broth of a newly isolated actinomycete by its antibacterial activity. A novel isolation method was developed and crystalline product was obtained in good yield. The structure was determined by spectroscopic examination of the product and its acetyl and trimethylsilyl derivatives. The racemic form of this compound had already been synthezised by G. E. McCasland et. al., as analog of galactose.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 25 (1983), S. 1509-1519 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cellulose fermentation studies were conducted with a thermotolerant strain of Aspergillus terreus. Batch cultivation of A. terreus using purified or complex cellulose showed that 80-88% of the available cellulose was utilized in 30-36 h with an average doubling time of 7.5-8.3 h. The protein content in the biomass ranged from 23 to 38%. Semicontinuous cultivation studies, in which 90% of the biomass was withdrawn at the end of growth cycle, indicated that 84% of added cellulose was utilized with the biomass containing 32% crude protein. No loss in cellulose consumption, growth rate, or protein production occurred through two growth cycles. Continuous cultivation of A. terreus showed that 78-84% cellulose consumption occurred over growth temperatures ranging from 35 to 45°C. Maximum specific growth rates (0.14 h-1) occurred at 40 and 45°C with a minimum doubling time of 4.9 h.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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