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Direct spectroscopic determination of functional sulphydryl groups on intact cell surfaces by surface-enhanced resonance Raman scattering (1992)

Rospendowski, B. N. ; Campbell, J. M. ; Reglinski, J. ; [et al.]

Springer

European biophysics journal 21 (1992), S. 257-261

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ISSN: 1432-1017

Keywords: Surface-enhanced resonance Raman scattering (SERRS) ; Intact cells ; Erythrocyte ; Membrane proteins ; Bio-engineering

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Physics

Notes: Abstract Semi-quantitative and direct determination of labelled sulphydryl groups on the surface of intact erythrocytes has been accomplished for the first time with surface-enhanced resonance Raman scattering (SERRS). The method, which involves the use of citrate-reduced silver colloids, is sensitive and selective. A 10−8 M effective concentration of picomole quantities of sulphydryl groups was determined in the presence of the normally overwhelming signal from haemoglobin. This seminal study suggests that SERRS may be applied to other in situ, site-directed labelling experiments.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00185120

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An apparatus for the culture of algae under controlled conditions (1959)

Fogg, G. E. ; Smith, W. E. E. ; Miller, J. D. A.

New York, NY [u.a.] : Wiley-Blackwell

Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 59-76

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ISSN: 0368-1467

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: An apparatus is described in which large numbers of algal cultures can be maintained under the following conditions: (1) Constant temperature within the range 5 to 70°C, four different temperatures being maintained simultaneously; (2) uniform illumination at intensities up to 20,000 metre-candles; (3) continuous shaking at variable speed; and (4) aeration under aseptic conditions with mixtures of up to four different gases in any desired proportions.Among other features, thermoregulator heads, flow-meters and heaters for sterile filters, of improved design are described.To illustrate the use of the apparatus, data showing the effects of light intensity and temperature on the growth of Monodus subterraneus are presented.

Additional Material: 8 Ill.