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  • Biomass N  (1)
  • Key words: Mast cells — Antigen-mediated stimulation — Myosin I  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Seasonal changes in microbial biomass in soils cropped with palmarosa (Cymbopogon martinii L.) and Japanese mint (Mentha arvensis L.) in subtropical India (1995)
    Springer
    Biology and fertility of soils 19 (1995), S. 193-196 
    Details
    ISSN: 1432-0789
    Keywords: Microbial biomass C ; Biomass N ; Biomass P ; Seasonal changes ; Palmarosa ; Mentha arvensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Changes in microbial C, N, and P were investigated for 1 year in two soils with similar physicochemical properties but supporting different crops under subtropical conditions. One was cropped with palmarosa (Cymbopogon martinii L.) and the other with Japanese mint (Mentha arvensis L.). Both the season and the type of cropping had a significant influence on changes in the soil microbial biomass. In general, soil microbial biomass C, N, and P were highest in summer months and lowest in midwinter. Soil microbial biomass levels and microbial C:N and C:P ratios were higher and N:P ratios lower under palmarosa soil than under mint.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00336158
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Loss of cell surface microvilli on rat basophilic leukaemia cells precedes secretion and can be mimicked using the calmodulin antagonist trifluoperazine (1997)
    Springer
    Inflammation research 46 (1997), S. 354-360 
    Details
    ISSN: 1420-908X
    Keywords: Key words: Mast cells — Antigen-mediated stimulation — Myosin I
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Objective: We studied changes in cell surface morphology following treatment with secretagogue or trifluoperazine in a mast cell model.¶Materials and Methods: Rat basophilic leukaemia (RBL) cells were treated with antigen and or the calmodulin antagonist, 0–50 μM trifluoperazine (TFP). The release of a secretory granule enzyme, β-hexosaminidase, into the external medium was used as a measure of secretion. Quantitation of cell surface microvilli was determined by using a computer with input from a digitising tablet from scanning electron micrographs. Cytoskeletal proteins present in microvilli were analysed by confocal immunofluorescence.¶Results: When RBL cells are stimulated to secrete with an antigen, the cell surface is transformed from a microvillous morphology to a ruffled one. The cell surface rearrangement preceded β-hexosaminidase secretion: the majority of microvilli disappeared rapidly after stimulation (t1/2 of 39 s) whereas secretion can only be measured after a lag of 47 s. The calmodulin antagonist, TFP did not inhibit antigen-induced secretion or loss of microvilli, however TFP alone caused a similar loss of microvilli but was unable to stimulate or potentiate secretion. The microvilli mostly disappeared within 30 s, and a half-maximal effect occurred at approximately 8 μM TFP. Using immunofluorescence, calmodulin was localized to punctate structures on the dorsal cell surface which presumably correspond to the microvilli, and which also stained for F-actin and myosin I.¶Conclusions: Loss of cell surface microvilli on RBL cells precedes secretion and could reflect a cytoskeletal rearrangement which facilitates fusion of secretory granules with the membrane. It can be mimicked using trifluoperazine and we suggest it may involve calmodulin-binding components of the microvillus cytoskeleton such as myosin I.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000110050201
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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