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  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructural demonstration of calcitonin in osmium-fixed human medullary carcinoma of thyroid by the protein A-colloidal gold technique (1985)
    Springer
    Virchows Archiv 407 (1985), S. 407-417 
    Details
    ISSN: 1432-2307
    Keywords: Thyroid medullary carcinoma ; Calcitonin ; EC granule ; Serotonin ; Protein A-colloidal gold
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In two medullary carcinomas of the thyroid gland two types of secretory granules were found electron microscopically in the cytoplasm of the tumour cells. The sizes of the granules in one case ranged 103–345 nm in diameter; they were round in shape, and they co-existed in the same tumour cell. They could not, therefore, be distinctively subdivided into two types. In another case, secretory granules in the cytoplasm closely resemble EC granule in morphology. Using the protein A-colloidal gold (PAG) technique the content of secretory granules could be identified as calcitonin irrespective of their sizes or morphology. Immunoreactivity at the ultrastructural level was fairly well preserved even in the osmium-fixed tumour cells. The labelling index, expressed as a mean number of gold particles per unit square area of the secretory granule, was higher in the non-osmium-fixed tumour cells than in the osmium-fixed. Non-osmium-fixed tumour cells embedded either in epoxy or methacryl resin were almost equally labelled with gold particles. The result indicates that the PAG method is practicable to demonstrate the ultrastructural localization of calcitonin even in the osmium-fixed, epoxy resin embedded material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00709987
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Preclinical detection of men 2A gene carrier using linked DNA markers (1991)
    Springer
    Journal of human genetics 36 (1991), S. 165-170 
    Details
    ISSN: 1435-232X
    Keywords: MEN 2A ; gene carriers ; linked DNA markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We have performed preclinical risk estimation of multiple endocrine neoplasia type 2A (MEN 2A) by using the polymorphic DNA markers tightly linked to the MEN2A locus. The gene for MEN 2A has been assigned to the pericentromeric region of chromosome 10 by linkage analysis. The preclinical detection of gene carriers in MEN 2A families using tightly linked DNA markers is useful for surgical treatment at an early stage. The DNA markers, RBP3 (retinol-binding protein 3, interstitial) and FNRB (fibronectin receptor, beta polypeptide), are both tightly linked to the MEN2A locus, and are localized to opposite sides of the MEN2A locus. We have used RBP3 and FNRB as markers for preclinical diagnosis. Of 18 Japanese families with MEN 2A, 6 families are informative for both loci, and other 10 families are informative for either RBP3 or FNRB. In one informative family, a 20-year-old female is predicted to be the gene carrier (probability; about 99%). She should be carefully followed up till full penetrance age. We conclude that DNA-based prediction of MEN 2A is an effective procedure for clinical use.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01876580
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Human surface electrogastrograms: AC and DC measurements (1984)
    Springer
    Annals of biomedical engineering 12 (1984), S. 319-333 
    Details
    ISSN: 1573-9686
    Keywords: Electrogastrogram ; Electrodes ; Biopotentials ; Models
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Both ac and dc abdominal electrical recordings were made on fasted and fed human subjects using a spatial array of 18 cutaneous, biopotential electrodes. Controlled skin puncture minimized cutaneous potentials. Pregelling electrodes minimized drift. The recording sessions were between two and three hours in length. We determined the optimal location for ac recording of a 3 cpm wave at a location on the midline approximately 5 cm below the xiphoid process. Seven cpm waves were also observed in one individual at the same location. The dc values were fed into a Hewlett Packard 1000 computer which interpolated the intermediate values and plotted twodimensional contour plots which were examined visually. The right abdominal surface was typically 1.8 mV more negative than the left for prefeeding. The left abdominal surface was typically 4.6 mV more negative than the right for postfeeding. We propose an electrical model that explains these results during the fasted state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02407776
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    Paper (German National Licenses)
    Fulltext
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