ISSN:
1432-2013
Keywords:
Redox state of cytochromes
;
Blood-free brain perfusion
;
Reflection photometry
;
Nonlinear multicomponent analysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract After developing a blood-free perfused preparation of the guinea pig brain a nonlinear multicomponent analysis (NLMCA) of the reflection spectra of the brain surface was worked out by which the redox state of cytochromeaa 3;c andb could be evaluated quantitatively and almost continuously. The NLMCA allowed to simulate the tissue reflection spectra in such a way that only a small statistical error remained between the recorded and the computed spectra. Using redox states of cytochromeaa 3 andc as an intracellular oxygen probe it was found that in order to supply the guinea brain with sufficient oxygen by a saline medium equilibrated with 95% oxygen and 5% CO2 the temperature had to be reduced to 18–20°C. At this temperature cytochromeaa 3 was 95% oxidized.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00584764
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