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  • Bradyrhizobium  (2)
  • Glycine (root nodules)  (2)
  • Diaminobenzidine (DAB)  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Immunogold localization of nodule-specific uricase in developing soybean root nodules (1986)
    Springer
    Planta 167 (1986), S. 425-436 
    Details
    ISSN: 1432-2048
    Keywords: Bradyrhizobium ; Glycine (uricase in nodules) ; Immunogold labeling ; Nodulin ; Peroxisome biogenesis ; Root nodules ; Uricase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immunogold labeling was used to study the time of appearance and distribution of a nodule-specific form of uricase (EC 1.7.3.3) in developing nodules of soybean (Glycine max (L.) Merr.) inoculated with Bradyrhizobium japonicum. The enzyme was detected in thin sections of tissue embedded in either L R White acrylic resin or Spurr's epoxy resin, by employing a polyclonal antibody preparation active against a subunit of soybean nodule uricase. Antigenicity was better preserved in L R White resin, but ultrastructure was better maintained in Spurr's. Uricase was first detectable with protein A-gold in young, developing peroxisomes in uninfected cells, coincident with the release of Bradyrhizobium bacteroids from infection threads in adjacent infected cells. As the peroxisomes enlarged, labeling of the dense peroxisomal matrix increased. Gold particles were never observed over the paracrystalline inclusions of peroxisomes, however. Despite a close association between enlarging peroxisomes and tubular endoplasmic reticulum, uricase was not detectable in the latter. In mature nodules, labeling of uricase was limited to the large peroxisomes in uninfected cells. Small peroxisome-like bodies present in infected cells did not become labeled.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391217
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Spatial relationships between uninfected and infected cells in root nodules of soybean (1985)
    Springer
    Planta 165 (1985), S. 446-454 
    Details
    ISSN: 1432-2048
    Keywords: Glycine (root nodules) ; Nodule ; Root nodules (uninfected cells)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In soybean (Glycine max (L.) Merr.) the uninfected cells of the root nodule are responsible for the final steps in ureide production from recently fixed nitrogen. Stereological methods and an original quantitative method were used to investigate the organization of these cells and their spatial relationships to infected cells in the central region of nodules of soybean inoculated with Rhizobium japonicum strain USDA 3I1B110 and grown with and without nitrogen (as nitrate) in the nutrient medium. The volume occupied by the uninfected tissue was 21% of the total volume of the central infected region for nodules of plants grown without nitrate, and 31% for nodules of plants grown with nitrate. Despite their low relative volume, the uninfected cells outnumbered the much larger infected cells in nodules of plants grown both without and with nitrate. The surface density of the interface between the ininfected and infected tissue in the infected region was similar for nodules in both cases also, the total range being from 24 to 26 mm2/mm3. In nodules of plants grown without nitrate, all sampled infected cells were found to be in contact with at least one uninfected cell. The study demonstrates that although the uninfected tissue in soybean nodules occupies a relatively small volume, it is organized so as to produce a large surface area for interaction with the infected tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00398089
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The occurrence of leghemoglobin protein in the uninfected interstitial cells of soybean root nodules (1988)
    Springer
    Planta 175 (1988), S. 442-451 
    Details
    ISSN: 1432-2048
    Keywords: Bradyrhizobium ; Glycine (root nodules) ; Leghemoglobin ; Root nodule
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The distribution of leghemoglobin (Lb) in resin-embedded root nodules of soybean (Glycine max (L.) Merr.) was investigated using immunogold labeling. Using anti-Lb immunoglobulin G and protein A-gold, Lb or its apoprotein was detected both in cells infected by Bradyrhizobium japonicum and in uninfected interstitial cells. Leghemoglobin was present in the cytoplasm, exclusive of the organelles, and in the nuclei of both cell types. In a comparison of the density of labeling in adjacent pairs of infected and uninfected cells, Lb was found to be about four times more concentrated in infected cells. This is the first report of Lb in uninfected cells of any legume nodule; it raises the possibility that this important nodule-specific protein may participate in mediating oxygen flow to host plant organelles throughout the infected region of the nodule.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393063
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Cytochemical localization of uricase and catalase in developing root nodules of soybean (1987)
    Springer
    Protoplasma 140 (1987), S. 1-12 
    Details
    ISSN: 1615-6102
    Keywords: Cerium chloride ; Diaminobenzidine (DAB) ; Peroxisomes ; Root-nodules ; Soybean ; Uricase cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two different cytochemical methods were used to study the localization of uricase (EC 1.7.3.3) and catalase (EC 1.11.1.6) in developing root nodules of soybean (Glycine max) inoculated as seeds withBradyrhizobium japonicum. One of the methods employs DAB (3,3′-diaminobenzidine) and detects uricase activity indirectly by coupling it to endogenous catalase activity. The other method utilizes cerium chloride to detect uricase activity directly. These methods were modified to obtain not only a strong staining reaction but also improved ultrastructural preservation. With the indirect DAB method, intense staining indicative of both uricase and catalase activity was obtained in the enlarged peroxisomes of older uninfected cells. Similar staining was observed in enlarging peroxisomes of younger uninfected cells, and in the material of associated sacs whose bounding membranes appear to arise as distensions of the ER. The observations are discussed in relation to the controversial role of the ER in peroxisome biogenesis. Although the small peroxisome-like organelles of infected cells did not give a clearly positive reaction in the indirect DAB method, they reacted positively in the cerium chloride method, and are considered to be peroxisomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01273250
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    Paper (German National Licenses)
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