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Studies of lectin receptors of rat microglia in culture: receptor distribution and internalization (1999)

Wu, C. H. ; Yeh, S. T. ; Ling, E. A.

Springer

Experimental brain research 124 (1999), S. 89-99

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ISSN: 1432-1106

Keywords: Key words Microglial culture ; Brain macrophages ; Isolectin ; Ultrastructure ; Intracellular pathway

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract The present study examined the lectin labeling of diverse morphological forms of microglia in culture. Similar to amoeboid microglial cells in vivo, polymorphic microglia showed lectin labeling at their plasma membranes, as well as in a few cytoplasmic vesicles and vacuoles. This labeling pattern was observed in cultured microglia incubated with isolectin at 4°C for 30 min. Five minutes after the temperature was raised to 37°C, the surface lectin receptors appeared to be internalized, as shown by the occurrence of many subsurface lectin-labeled vesicles, vacuoles and tubule-like structures. With longer incubation (up to 1–2 h at 37°C), many lysosomes and a few trans-Golgi saccules and associated lysosome-like structures became labeled. Concomitant with these changes was a reduction of lectin labeling at the plasma, with labeling having vanished in most of the cells after 1–2 h of incubation. By 24 h, only a few cells retained surface lectin labeling. It appears, therefore, that irrespective of morphology, lectin labeling (including its intracellular pathway) of microglia in culture parallels that of amoeboid microglia in vivo. This would offer a useful model for the study of lectin turnover in microglia and help to explain the roles of such receptors in microglial differentiation and function.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s002210050603

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The purification of lithium and its analysis for hydrogen and nitrogen (1975)

Blair, A. J. ; Wu, C. H.

Springer

Fresenius' Zeitschrift für analytische Chemie 275 (1975), S. 261-263

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ISSN: 1618-2650

Keywords: Best. von Wasserstoff in Lithium ; Massenspektrometrie ; Vakuumdestillation ; Best. von Stickstoff in Lithium ; Photometrie ; Vakuumdestillation

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Description / Table of Contents: Summary For the purification of lithium and determination of its H and N content the sample is distilled in vacuum at 650–700° C in a stainless steel apparatus, Li collected in molybdenum crucibles and analysed for H by mass spectrometry. N is determined chemically by a colorimetric method. The limit of detection is 0.5 and 10 ppm for H and N, respectively.

Notes: Zusammenfassung Die Probe wird im Vakuum bei 650–700° C in einer Apparatur aus rostfreiem Stahl destilliert, Li in einem Molybdäntiegel aufgefangen und H massenspektrometrisch bestimmt. N wird chemisch mit Hilfe einer colorimetrischen Methode bestimmt. Die Nachweisgrenze liegt bei 0,5 ppm (H) bzw. 10 ppm (N).