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  • 1
    Electronic Resource
    Electronic Resource
    Molecular cloning of protease gene(s) from Xanthomanas campestris pv. campestris: Expression in Escherichia coli and role in pathogenicity (1987)
    Springer
    Molecular genetics and genomics 210 (1987), S. 443-448 
    Details
    ISSN: 1617-4623
    Keywords: Cloning ; Xanthomonas ; Protease ; Pathogenicity ; Brassica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The recombinant plasmid pIJ3070 isolated from a genomic library of Xanthomonas campestris pv. campestris constructed in the conjugal cosmid pLAFR3 contains protease gene(s) which can be expressed in Escherichia coli. Tn5 mutagenesis and subcloning revealed that the protease structural gene(s) is(are) located in a ca. 10 kb EcoRI fragment. Several protease-minus mutants of X. c. campestris were obtained by Tn5 mutagenesis of pIJ3070 and marker exchange techniques. Studies of pathogenicity of these Tn5 mutants showed that the protease is not critically important for the pathogenicity of X. c. campestris on turnip plants but may play a minor role in disease development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327195
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A mutation that blocks exopolysaccharide synthesis prevents nodulation of peas by Rhizobium leguminosarum but not of beans by R. phaseoli and is corrected by cloned DNA from Rhizobium or the phytopathogen Xanthomonas (1986)
    Springer
    Molecular genetics and genomics 203 (1986), S. 320-323 
    Details
    ISSN: 1617-4623
    Keywords: Exopolysaccharide ; Legume ; Nodules ; Rhizobium ; Xanthomonas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A Tn5-induced mutant strain of R. phaseoli which failed to synthesize exopolysaccharide (EPS) was isolated and was shown to induce normal nitrogen-fixing nodules on Phaseolus beans, the host of this Rhizobium species. The corresponding wild-type Rhizobium DNA was cloned in a wide host-range vector and by isolating Tn5 insertions in this cloned DNA, mutations in a gene termed pss (polysaccharide synthesis) were isolated. These were introduced by marker exchange into near-isogenic strains of R. leguminosarum and R. phaseoli which differed only in the identity of their symbiotic plasmids. Whereas the EPS-deficient mutant strain of R. phaseoli induced normal nitrogen-fixing nodules on Phaseolus beans, the same mutation prevented nodulation of peas by a strain of R. leguminosarum which normally nodulates this host. Further, it was found that DNA cloned from the plant pathogen Xanthomonas campestris pathover campestris could correct the defect in EPS synthesis in R. leguminosarum and R. phaseoli and also restored the ability to nodulate peas to the pss::Tn5 mutant strain of R. leguminosarum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333974
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Interaction of Xanthomonas with crucifers (1992)
    Springer
    European journal of plant pathology 98 (1992), S. 147-155 
    Details
    ISSN: 1573-8469
    Keywords: Arabidopsis ; Brassica ; enzymes ; hrp genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract General studies of the bacterial pathogenXanthomonas campestris have revealed many classes of gene which are essential for pathogenicity to cruciferous plants. ‘Pathogenicity genes’ include structural genes encoding extracellular plant-degrading enzymes, genes required for the export (or secretion) of the enzymes from the bacterial cell, several independent sets of regulatory genes, a cluster ofhrp genes, and several genes of unknown function. Plants respond to infection by synthesizing characteristic pathogenesis-related proteins. The spectrum of proteins produced depends on the genotype of the challenging bacterium.Arabidopsis is susceptible to infection byXanthomonas, which should make it feasible to analyze plant resistance and response by molucular genetic techniques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01974481
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    Paper (German National Licenses)
    Fulltext
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