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  • 1
    Electronic Resource
    Electronic Resource
    Fluorescent banding pattern analysis of eight taxa of Phaseolus and Vigna in relation to their phylogenetic relationships (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 38-43 
    Details
    ISSN: 1432-2242
    Keywords: Fluorescent chromosome banding patterns ; Phaseolus ; Vigna ; Phylogenetic relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phylogenetic relationships among eight taxa of seven species of Phaseolus and Vigna (Phaseolus angularis, P. aureus, P. calcaratus, P. coccineus, P. vulgaris, Vigna sesquipedalis and V. sinensis; 2n = 22 each) were studied by the fluorescent chromosome banding technique. Preparations of somatic metaphase chromosomes of each taxon were sequentially stained with Giemsa, GC-specific fluorochrome chromomycin A3 (CMA) and AT-specific fluorochrome 4′-6-diamidino-2-phenylindole (DAPI). On the basis of the fluorescent banding patterns of the 22 chromosomes of each taxon, P. angularis, P. coccineus (from China and Korea) and P. vulgaris were grouped into one group (“Phaseolus group”), P. aureus and two Vigna species were grouped into another (“Vigna group”) and P. calcaratus was grouped in an independent group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223741
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  • 2
    Electronic Resource
    Electronic Resource
    Interspecific hybrid plant formation by electrofusion in Nicotiana (1987)
    Springer
    Theoretical and applied genetics 75 (1987), S. 1-4 
    Details
    ISSN: 1432-2242
    Keywords: Electrofusion ; Fertile somatic hybrid ; Fraction I protein analysis ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We obtained complete hybrid plants by electrofusion of mesophyll protoplasts from Nicotiana glauca and N. langsdorffii. Electrofocusing analysis of Fraction I proteins isolated from the leaves of these plants confirmed their hybridity. Cytological analysis indicated that the chromosome number (2n) of these plants is between 60 to 66, suggesting that they are the products of triple fusion. All plants were fertile and set viable seeds after self pollination. As we did not use an AC field for electrofusion, the present results indicate that an AC field is not essential for obtaining hybrid plants with electrofusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249133
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of a highly repeated DNA sequence and its chromosomal localization in Allium fistulosum (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 312-316 
    Details
    ISSN: 1432-2242
    Keywords: Allium fistulosum ; Repeated DNA sequence ; In situ hybridization ; Nucleotide sequence ; C-banding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A highly repeated DNA sequence with a repeating unit of approximately 380bp was found in EcoRV digests of the total genomic DNA of Allium fistulosum. Three independent clones containing this unit were isolated, and their repeating units sequenced. These units showed more than 94% sequence homology, and the copy number was estimated to be about 2.8×106 per haploid genome. In situ hybridization, with the repeating unit as a probe, and C-banding analyses indicated that the repeated DNA sequence of A. fistulosum is closely associated with the major C-heterochromatin in the terminal regions of all 16 chromosomes at mitotic metaphase. The characters of the repeating unit are similar to those of the A. cepa unit, which is taxonomically closely related to A. fistulosum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221970
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    Paper (German National Licenses)
    Fulltext
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