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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 65 (1983), S. 145-147 
    ISSN: 1432-2242
    Keywords: Diploid wheat ; Nucleolus organiser ; In situ hybridisation ; Telocentric markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The two nucleolus organiser chromosomes of diploid wheat are identified as 1A and 5A by the combination of in situ hybridisation and cytological markers.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 65 (1983), S. 343-348 
    ISSN: 1432-2242
    Keywords: C-banding ; Aegilops ; Intraspecific variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intraspecific variation in C-banding patterns can be used to differentiate subspecies of Ae. comosa, i.e. eu-comosa and heldrechii, but not those belonging to Ae. speltoides. Among the eu-comosa accessions, comosa E appears to possess a totally different karyotype as well as banding pattern. Two contrasting types of polymorphic changes were found. The first and more common type, observed in the 2 subspecies of Ae. speltoides and 2 accessions of Ae. comosa ssp. eucomosa, involved variation in C-band size and presence or absence of interstitial, terminal and proximal bands within a basic recognisable pattern. The second type involved complete repatterning of C-bands and could be seen in Ae. comosa where the ssp. eu-comosa has predominantly interstitial C-bands in contrast to centromeric and telomeric bands in the other ssp. heldrechii. Furthermore, the extent of polymorphic variation was found to vary between chromosomes of Ae. speltoides. That polymorphic changes have occurred without loss of fertility in hybrids between subspecies of each of the 2 species confirms that these sorts of changes have no effect on chromosome pairing or fertility. Polymorphic changes appear to be widespread within and between diploid Aegilops species and their non-random distribution seem to suggest that these changes could perhaps be intimately associated with the processes of speciation and subspeciation.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 58 (1980), S. 97-100 
    ISSN: 1432-2242
    Keywords: Nucleolus organizers ; In situ hybridisation ; Diploid wheats ; Ribosomal RNA genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Labelled RNA, transcribed in vitro from wheat ribosomal DNA cloned in a bacterial plasmid, has been hybridised to metaphase chromosomes of five diploid wheats. Autoradiography of the chromosomes has provided unequivocal evidence that these genotypes possess two pairs of nucleolus organizer chromosomes. The diploid wheat accessions used possess widely differing numbers of ribosomal RNA genes.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 61 (1982), S. 27-33 
    ISSN: 1432-2242
    Keywords: Preferential transmission ; In situ hybridization ; C-banding ; Aegilops sharonensis ; Wheat ; Addition ; Substitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An attempt to produce a set of addition lines of Aegilops sharonensis to the wheat variety ‘Chinese Spring’ produced only one addition line. This was due to preferential transmission of one chromosome from Ae. sharonensis. This chromosome was studied in detail by established cytological methods of chromosome observation and by the newer techniques of C-banding and in situ hybridization of a cloned DNA sequence. The chromosome was found to be partially homologous to an Ae. sharonensis chromosome of similar behaviour in another wheat addition line. The incomplete homology of the two Ae. sharonensis chromosomes was due to the presence of a translocated segment of a wheat chromosome. — Substitution lines of the Ae. sharonensis chromosome for wheat homoeologous group 4 were produced and the Ae. sharonensis chromosome thereby designated 4 S l .
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-2242
    Keywords: Triticum timopheevi ; Chromosomes ; C-banding ; In situ hybridisation ; Heterochromatin ; Translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The chromosomes of the tetraploid wheats Triticum timopheevi (Genome AAGG) and T. araraticum (Genome AAGG) were C-banded at mitosis. The identity of the banded and unbanded chromosomes was then established by firstly making comparisons with the hexaploid species T. zhukovskyi which has the genome formula AAAAGG. Secondly, the meiotic pairing in F1 hybrids between T. timopheevi and diploid wheats was examined by means of C-banding. The results showed that the banded chromosomes belonged to the G genome, while the unbanded chromosomes belonged to the A genome. Only one of the two pairs of satellited chromosomes had strong heterochromatic bands. The relationship between the genomes of T. timopheevi and T. dicoccum (Genome AABB) was then assessed at meiosis in hybrids between these species, using the techniques of C-banding and in situ hybridisation of a cloned ribosomal RNA gene probe. It was concluded that there were differences both in the amount and distribution of heterochromatin and also translocation differences between the species.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 61 (1982), S. 285-288 
    ISSN: 1432-2242
    Keywords: Nucleolar organisers ; In situ hybridisation ; Tetraploid and hexaploid wheats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The technique of in situ hybridisation of cloned ribosomal DNA has been used to establish the numbers of nucleolar organising sites in a range of tetraploid and hexaploid wheats.
    Type of Medium: Electronic Resource
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