ISSN:
1432-2242
Keywords:
Wheat
;
Lophopyrum
;
Translocation
;
Repetitive DNA sequence
;
C-banding
;
In situ hybridization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Efforts to transfer wheat curl mite (Eriophyes tulipae Keifer) resistance from Lophopyrum ponticum 10X (Podb.) Love to bread wheat (Triticum aestivum L.) have resulted in the production of a number of cytogenetic stocks, including an addition line of 6Ag, a “ditelo” addition line, and a wheat-Lophopyrum translocation line. Characterization of these lines with C-banding, in situ hybridization with a Lophopyrum species-specific repetitive DNA probe (pLeUCD2), and Southern blotting with pLeUCD2 and a 5S ribosomal DNA probe (pScT7) confirmed that the distal portion of the short arm of 6Ag was translocated onto the distal portion of 5BS (5BL. 5BS-6AgS). It was also determined that the “ditelo” addition was an acrocentric chromosome of 6AgS.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00212608
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