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  • CAD-EBES network  (1)
  • Gel-permeation chromatography  (1)
  • Key words. Late replication banding; DAPI; actinomycin D; frogs.  (1)
  • Malignant transformation  (1)
Source
Material
Years
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Technologies for electron beam direct writing
    Amsterdam : Elsevier
    Microelectronic Engineering 1 (1983), S. 69-90 
    Details
    ISSN: 0167-9317
    Keywords: CAD-EBES network ; EB direct writing ; EB technology
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Electrical Engineering, Measurement and Control Technology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-9317(83)90013-8
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Immunohistochemical localisation of stem cell factor (SCF) with comparison of its receptor c-Kit proto-oncogene product (c-KIT) in melanocytic tumours (1995)
    Springer
    Virchows Archiv 427 (1995), S. 283-288 
    Details
    ISSN: 1432-2307
    Keywords: c-KIT ; Immunohistochemistry ; Malignant transformation ; Melanocyte ; SCF
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In order to characterise the distribution and role of stem cell factor (SCF), a recently-reported growth factor for normal melanocytes, we carried out an immunohistochemical study on benign and malignant melanocytic tumours with a comparison with the presence of its receptor c-Kit proto-oncogene product (c-KIT). In normal skin, SCF was mainly observed in endothelial cells of blood vessels but not frequently in basal melanocytes, whereas c-KIT was predominantly localised in tissue mast cells. In benign neoplastic melanocytes (common melanocytic naevi), localisation of SCF and c-KIT was complementary: SCF was mostly found in dermal naevus cells while c-KIT was revealed in epidermal naevus cells, although the expression of the latter antigen was not frequent. Malignant melanoma cells showed less frequent expression of these antigens than those in benign lesions. Of five cultured melanoma cell lines, SCF was observed in only one, and c-KIT was not found in any melanoma cells. No quantitative or qualitative alterations assessed by Western blot analysis were induced in the presence of phenotypic modifiers (sodium butyrate and HMBA). Present data suggest that loss of SCF expression in neoplastic melanocytes is commonly associated with malignant transformation of pigment cells rather than loss of its receptor c-KIT.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00203396
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Fluorescence replication banding of frog chromosomes (1997)
    Springer
    Cellular and molecular life sciences 53 (1997), S. 73-77 
    Details
    ISSN: 1420-9071
    Keywords: Key words. Late replication banding; DAPI; actinomycin D; frogs.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. To identify individual chromosomes of a frog karyotype by their fluorescence banding patterns, chromosomes were stained with actinomycin D and 4,6-diamidino-2-phenylindole (DAPI) after incorporation of BrdU during the late S-phase. The chromosomes of three Rana species which were selected for this study (R. ridibunda, R. lessonae and R. japonica) showed well-defined late replication bands. The fluorescence patterns obtained were the reverse of those produced by a 4Na-EDTA Giemsa-staining technique. Fluorescence patterns of the two water frog species (R. ridibunda and R. lessonae) were similar to each other, except for the different fluorescence of the centromeric heterochromatin, which gave extremely bright signals in R. ridibunda but no signal in R. lessonae. Experiments also showed differences between the fluorescence patterns of R. lessonae chromosome 13 in the Italian and Luxembourgian populations. These results sho w that the fluorescence replication banding using actinomycin D and DAPI is very effective in identifying individual frog chromosomes and detecting their structural changes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00000582
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation of the mixed ligand complex of beryllium(II) with acetylacetone and thenoyltrifluoroacetone by gel permeation chromatography (1981)
    Springer
    Chromatographia 14 (1981), S. 189-191 
    Details
    ISSN: 1612-1112
    Keywords: Gel-permeation chromatography ; Beryllium complexes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The mixed ligand, metal complex (acetylacetonato) (thenoryltrifluoroacetonato) beryllium(II) has been isolated from a reaction mixture of bis(acetylacetonato) beryllium(II) and bis(thenoyltrifluoroacetonato)beryllium(II) in ethyl acetate by gel permeation chromatography using Shodex 801 gel column and ethyl acetate as the solvent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02260150
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    Paper (German National Licenses)
    Fulltext
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