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  • CAM 5.2 antibody  (1)
  • Striatal neurons ATP-sensitive K+ channels  (1)
  • ion association  (1)
  • 1
    Electronic Resource
    Electronic Resource
    ATP-sensitive potassium channels in freshly dissociated adult rat striatal neurons: activation by metabolic inhibitors and the dopaminergic receptor agonist quinpirole (2000)
    Springer
    Pflügers Archiv 440 (2000), S. 530-547 
    Details
    ISSN: 1432-2013
    Keywords: Striatal neurons ATP-sensitive K+ channels
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The characteristics of adenosine 5′-triphosphate (ATP)-sensitive K+ channels in acutely isolated striatal neurons from adult rats were examined. Neurons had a resting membrane potential of –53.9±1.2 mV (n=66), with evoked or spontaneous action potentials firing at 10±0.7 Hz, and large inwards and outwards whole-cell currents. In cell-attached patches with a high [K+] in the pipette, a voltage-independent, ATP-insensitive 16.5±1.5 pS channel was observed in 375 out of 452 cells. Bath application of Na+-azide (0.5–2 mM) to 108 neurons revealed another 145.7±3.5 pS (LKATP) channel in 65 neurons; this channel was blocked by tolbutamide. The LKATP channel exhibited a high open probability (P o, 0.8±0.05) at 0 mV pipette potential. Varying the pipette [K+] shifted the reversal potential of LKATP, showing the channel's K+ selectivity. Cytoplasmic ATP (ATPi) reversibly inhibited LKATP, with an inhibitory constant (K i) of 0.12 mM. LKATP was sensitive to intracellular Ca2+ but insensitive to iberiotoxin. In 25% of cell-attached patches, the presence of quinpirole in the pipette opened a third type of channel (90.6±1.7 pS, termed D2KATP). Sulpiride, a dopamine D2-receptor antagonist, inhibited D2KATP. ATPi reversibly inhibited D2KATP, with a K i of 0.212 mM. The Na+-azide- or quinpirole-induced current caused a tolbutamide-sensitive membrane hyperpolarization and a marked reduction in action potential frequency. We propose that ATP-sensitive K+ channels play a metabolism-dependent role in striatal neurons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240000322
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Study of ion solvation and ion association in polymer gel electrolyte with polyethylene oxide-co-polypropylene oxide as a plasticizer (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Polymer International 41 (1996), S. 301-305 
    Details
    ISSN: 0959-8103
    Keywords: gel electrolyte ; polytriethylene glycol dimethacrylate ; ion solvation ; ion association ; molal conductance ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Using a molal conductance method, ion solvation and ion association in polytriethylene glycol dimethacrylate (PTREGD)-LiClO4 gel electrolytes with amorphous ethylene oxide-co-propylene oxide (EO-co-PO, M̄n = 1750) as the plasticizer were investigated. It was found that the fraction of solute existing as single ions (αi) and ion pairs (αp) decreases, while that of triple ions (αt) increases linearly with increasing salt concentration. The dependence of these fractions on molecular weight of plasticizer was also examined. It was shown that αi and αt increase and αp decreases with increasing molecular weight. The result of temperature dependence of these fractions was very interesting: when the temperature is lower than 55°C, αi increases while αp and αt decrease with increasing temperature; however, when the temperature is higher than 55°C, the reverse is true.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pi.1996.210410312
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  • 3
    Electronic Resource
    Electronic Resource
    S-phase determination of immunoselected cytokeratin-containing breast cancer cells improves the prediction of recurrence (1994)
    Springer
    Breast cancer research and treatment 29 (1994), S. 179-187 
    Details
    ISSN: 1573-7217
    Keywords: breast cancer ; CAM 5.2 antibody ; cytokeratin ; flow cytometry ; prognosis ; S-phase fraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Estimation of S-phase fraction in breast carcinomas with single parameter flow cytometry may include errors due to dilution of cancer cells by host cells. Use of tissue specific markers may to some extent correct for the effect of dilution. S-phase fraction was estimated by flow cytometry with and without immunoselection in 80 DNA-euploid breast carcinomas in stage I-II. The tumor tissue was mechanically disintegrated and fixed in ethanol. A primary antibody, specific for cytokeratins 8 and 18, was added before incubation with the secondary FITC-conjugated antibody. S-phase fraction was calculated for both the gated (cytokeratin-positive) and the ungated cell population. An increasing proportion of tetraploid cells compared to diploid cells was found when the immunoselection method was used. The gated population tended to have a higher S-phase fraction than the ungated population. In univariate analysis S-phase fraction estimated from both ungated and gated cell populations yielded significant information for predicting recurrence when stratified for tumor size and nodal status. In bivariate analysis S-phase fraction of the gated population contributed prognostic information in addition to S-phase fraction of the ungated population when both variables were included in the analysis. Our conclusion is that S-phase fraction calculated from cytokeratin-positive cells provides prognostic information in addition to ungated S-phase values in DNA euploid breast carcinomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00665679
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    Paper (German National Licenses)
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