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  • Tomato  (2)
  • CAPS markers  (1)
  • Gene clustering Bulked segregant analysis  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Development of diagnostic PCR markers closely linked to the tomato powdery mildew resistance gene Ol-1 on chromosome 6 of tomato (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 918-924 
    Details
    ISSN: 1432-2242
    Keywords: Keywords Resistance ; Tomato powdery mildew ; Tomato ; Mapping ; Oidium lycopersicum ; RFLP ; Sequence characterised amplified region (SCAR)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Lycopersicon hirsutum G1.1560 is a wild accession of tomato that shows resistance to Oidium lycopersicum, a frequently occurring tomato powdery mildew. This resistance is largely controlled by an incompletely dominant gene Ol-1 near the Aps-1 locus in the vicinity of the resistance genes Mi and Cf-2/Cf-5. Using a new F2 population (n=150) segregating for resistance, we mapped the Ol-1 gene more accurately to a location between the RFLP markers TG153 and TG164. Furthermore, in saturating the Ol-1 region with more molecular markers using bulked segregant analysis, we were able to identify five RAPDs associated with the resistance. These RAPDs were then sequenced and converted into SCAR markers: SCAB01 and SCAF10 were L. hirsutum-specific; SCAE16, SCAG11 and SCAK16 were L. esculentum-specific. By linkage analysis a dense integrated map comprising RFLP and SCAR markers near Ol-1 was obtained. This will facilitate a map-based cloning approach for Ol-1 and marker-assisted selection for powdery mildew resistance in tomato breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051562
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    An integrated high-density RFLP-AFLP map of tomato based on two Lycopersicon esculentum×L. pennellii F2 populations (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 254-271 
    Details
    ISSN: 1432-2242
    Keywords: Key words Molecular markers ; Integrated linkage map ; Tomato ; Lycopersicon species ; AFLP ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Two independent F2 populations of Lycopersicon esculentum×L. pennellii which have previously been investigated in RFLP mapping studies were used for construction of a highly saturated integrated AFLP map. This map spanned 1482 cM and contained 67 RFLP markers, 1078 AFLP markers obtained with 22 EcoRI+MseI primer combinations and 97 AFLP markers obtained with five PstI+MseI primer combinations, 231 AFLP markers being common to both populations. The EcoRI+MseI AFLP markers were not evenly distributed over the chromosomes. Around the centromeric region, 848 EcoRI+ MseI AFLP markers were clustered and covered a genetic distance of 199 cM, corresponding to one EcoRI+ MseI AFLP marker per 0.23 cM; on the distal parts 1283 cM were covered by 230 EcoRI+MseI AFLP markers, corresponding to one marker per 5.6 cM. The PstI/MseI AFLP markers showed a more even distribution with 16 PstI/MseI AFLP markers covering a genetic distance of 199 cM around the centromeric regions and 81 PstI/MseI AFLP markers covering a genetic distance of 1283 cM on the more distal parts, corresponding to one marker per 12 and 16 cM respectively. In both populations a large number of loci showed a significant skewed segregation, but only chromosome 10 loci showed skewness that was similar for both populations. This ultra-dense molecular-marker map provides good perspectives for genetic and breeding purposes and map-based cloning.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051231
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  • 3
    Electronic Resource
    Electronic Resource
    Resistance to powdery mildew (Oidium lycopersicum) in Lycopersicon hirsutum is controlled by an incompletely-dominant gene Ol-1 on chromosome 6 (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 467-473 
    Details
    ISSN: 1432-2242
    Keywords: Lycopersicon hirsutum ; Resistance gene mapping ; Oidium lycopersicum ; Gene clustering Bulked segregant analysis ; Powdery mildew ; Tomato RAPD ; Ol-1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The inheritance of resistance to powdery mildew (Oidium lycopersicum) in Lycopersicon hirsutum was investigated by disease tests in segregating populations obtained by hybridising tomato (L. esculentum) cv Moneymaker with the wild relative L. hirsutum G1.1560. One incompletely dominant gene Ol-1 was found to largely control resistance to the disease. To map Ol-1, DNA pools from seven resistant and ten susceptible F2 plants were analyzed for random amplified polymorphic DNA (RAPD). With 32 primers tested, one RAPD, primed with the sequence 5′-GACGTGGTGA-3′, was observed between the susceptible and the resistant bulks, which cosegregated with resistance in the F2 population of L. esculentum × L. hirsutum G1.1560. This RAPD was mapped on chromosome 6 by using an F2 (L. esculentum × L. pennellii) already mapped for 49 RFLPs. RFLP analysis of the F2 from L. esculentum cv Moneymaker × L. hirsutum G1.1560 demonstrated that Ol-1 maps near the Aps-1 region on chromosome 6, in the vicinity of the resistance genes to Meloidogyne spp. (Mi) and to Cladosporium fulvum (Cf-2/Cf-5).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225382
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The Cf-ECP2 gene is linked to, but not part of, the Cf-4/Cf-9 cluster on the short arm of chromosome 1 in tomato (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 839-845 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsLycopersicon esculentum ; Cladosporium fulvum ; Pathogenicity ; CAPS markers ; Hcr9
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A gene has been identified in tomato, which confers resistance to Cladosporium fulvum through recognition of the pathogenicity factor ECP2. Segregation analysis of F2 and F3 populations showed monogenic dominant inheritance, as for previously reported Cf resistances. The gene has been designated Cf-ECP2. Using several mapping populations, Cf-ECP2 was accurately mapped on chromosome 1, 7.7 cM proximal to TG236 and 6.0 cM distal to TG184. Although Cf-ECP2 is linked to Cf-4, it is not located in the Hcr9 cluster “Milky Way”. Therefore, Cf-ECP2 is the first functional Cf homologue on chromosome 1 that does not belong to this Hcr9 cluster. No recombination events between Cf-ECP2 and CT116 have been observed in three populations tested, representing 282 individuals. The low value for the physical distance per cM around CT116 reported previously and the high probability that Cf-ECP2 is also a member of a Hcr9 cluster will facilitate cloning of the locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051148
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    Paper (German National Licenses)
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