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  • 1
    Electronic Resource
    Electronic Resource
    The Cf-ECP2 gene is linked to, but not part of, the Cf-4/Cf-9 cluster on the short arm of chromosome 1 in tomato (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 839-845 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsLycopersicon esculentum ; Cladosporium fulvum ; Pathogenicity ; CAPS markers ; Hcr9
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A gene has been identified in tomato, which confers resistance to Cladosporium fulvum through recognition of the pathogenicity factor ECP2. Segregation analysis of F2 and F3 populations showed monogenic dominant inheritance, as for previously reported Cf resistances. The gene has been designated Cf-ECP2. Using several mapping populations, Cf-ECP2 was accurately mapped on chromosome 1, 7.7 cM proximal to TG236 and 6.0 cM distal to TG184. Although Cf-ECP2 is linked to Cf-4, it is not located in the Hcr9 cluster “Milky Way”. Therefore, Cf-ECP2 is the first functional Cf homologue on chromosome 1 that does not belong to this Hcr9 cluster. No recombination events between Cf-ECP2 and CT116 have been observed in three populations tested, representing 282 individuals. The low value for the physical distance per cM around CT116 reported previously and the high probability that Cf-ECP2 is also a member of a Hcr9 cluster will facilitate cloning of the locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051148
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Tomato chromosome 1: high resolution genetic and physical mapping of the short arm in an interspecific Lycopersicon esculentum×L. peruvianum cross (1997)
    Springer
    Molecular genetics and genomics 253 (1997), S. 455-462 
    Details
    ISSN: 1617-4623
    Keywords: Key words Lycopersicon species ; Recombination frequency ; Genetic map ; Physical map ; High-molecular-weight DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A detailed map of part of the short arm of chromosome 1 proximal to the Cf-4/Cf-9 gene cluster was generated by using an F2 population of 314 plants obtained from the cross between the remotely related species Lycopersicon esculentum and L. peruvianum. Six markers that cosegregate in an L. esculentum×L. pennellii F2 population showed high recombination frequencies in the present interspecific population, spanning an interval of approximately 13 cM. Physical distances between RFLP markers were estimated by pulsed field gel electrophoresis of high-molecular-weight DNA and by identifying YACs that recognized more than one RFLP marker. In this region 1 cM corresponded to 55–110 kb. In comparsion with the value of 730 kb per cM averaged over the entire genome, this reflects the remarkably high recombination frequencies in this region in the hybrid L. esculentum×L. peruvianum progeny population. The present data underline the fact that recombination is not a process that occurs randomly over the entire genome, but can vary dramatically in intensity between chromosomal regions and among populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050343
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    Paper (German National Licenses)
    Fulltext
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