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  • CCK cholecystokinine  (1)
  • MOPS 3-[N-morpholino] propane sulfonic acid buffer  (1)
  • Microalgae  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Aquaculture international 4 (1996), S. 315-324 
    ISSN: 1573-143X
    Keywords: Bacteria ; Great scallop Pecten maximus ; Microalgae ; Nutrition ; Vitamins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Vitamins were analysed in food (microalgae) and larvae of great scallop, Pecten maximus, during larval development. Microalgae used to feed larvae in hatcheries show great variability in their vitamin composition depending on both the species and culture condition (phase of growth). The microalgae used to feed Pecten maximus larvae were rich in vitamins; their content compared with diets used in fish culture appeared sufficient, with the possible exceptions of pyridoxine, biotin and pantothenic acid. Vitamins in bacteria, isolated from the larval rearing tank were also analysed, as they can also contribute to the diet. Vitamin B12, α-tocopherol and Β-carotene were detected in very low concentration in bacteria; however, some bacterial strains were rich in pantothenic acid, and the pattern of other vitamins was similar to that from microalgae. The presence of bacteria can complement the diet in panthothenic acid, as it has been demonstrated that bacteria are ingested by larvae. The vitamin content of Pecten maximus larvae was analysed from the second day after hatching to just before metamorphosis. The content of some vitamins, ascorbic acid (C), α-tocopherol and Β-carotene, increased during larval development, suggesting that their requirement was satisfied. However, thiamin and riboflavin decreased during larval development and further studies, possibly using microencapsulated vitamins supplements, are needed to determine whether these vitamins are limiting during larval development.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 170 (2000), S. 21-26 
    ISSN: 1432-136X
    Keywords: Key words Amylase regulation ; Digestive enzymes ; Oyster ; Feeding stimulus ; Abbreviations bp base pair ; cDNA copy DNA ; CCK cholecystokinine ; EDTA ethylenediaminetetraacetic acid ; IGF insulin growth factor ; MOPS 3-[N-morpholino] propane sulfonic acid buffer ; PCR polymerase chain reaction ; pfu plaque forming unit ; SDS sodium dodecylsulfate ; SSC sodium citrate buffer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  A Crassostrea gigas digestive gland copy DNA (cDNA) library constructed in the λ phage ZapII (Stratagene, La Jola, USA) was screened with an amylase heterologous probe. To get access to the complete cDNA, a polymerase chain reaction extension was conducted using DNA extracted from the phages. The complete cDNA sequence is 1688 base pairs (EMBL = Y08370). The deduced protein sequence is 519 aminoacids long with a 19 aminoacid signal peptide. Similarity with Pectenmaximus amylase is 72%. A 3-day nutrition experiment with a cyclic algal food supply was carried out. Amylase enzyme activities and mRNAs were individually measured on five animals, nine times a day. Messenger RNAs were quantified by dot hybridization using the previously characterized cDNA as probe. Variation of amylase mRNA was observed, in relation with the level of activity of the enzyme. Coordinated changes in RNA and enzyme levels suggested a possible transcriptional regulation of amylase in C. gigas as in vertebrates.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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