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  • 1
    Electronic Resource
    Electronic Resource
    Amylase mRNA expression in Crassostrea gigas during feeding cycles (2000)
    Springer
    Journal of comparative physiology 170 (2000), S. 21-26 
    Details
    ISSN: 1432-136X
    Keywords: Key words Amylase regulation ; Digestive enzymes ; Oyster ; Feeding stimulus ; Abbreviations bp base pair ; cDNA copy DNA ; CCK cholecystokinine ; EDTA ethylenediaminetetraacetic acid ; IGF insulin growth factor ; MOPS 3-[N-morpholino] propane sulfonic acid buffer ; PCR polymerase chain reaction ; pfu plaque forming unit ; SDS sodium dodecylsulfate ; SSC sodium citrate buffer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  A Crassostrea gigas digestive gland copy DNA (cDNA) library constructed in the λ phage ZapII (Stratagene, La Jola, USA) was screened with an amylase heterologous probe. To get access to the complete cDNA, a polymerase chain reaction extension was conducted using DNA extracted from the phages. The complete cDNA sequence is 1688 base pairs (EMBL = Y08370). The deduced protein sequence is 519 aminoacids long with a 19 aminoacid signal peptide. Similarity with Pectenmaximus amylase is 72%. A 3-day nutrition experiment with a cyclic algal food supply was carried out. Amylase enzyme activities and mRNAs were individually measured on five animals, nine times a day. Messenger RNAs were quantified by dot hybridization using the previously characterized cDNA as probe. Variation of amylase mRNA was observed, in relation with the level of activity of the enzyme. Coordinated changes in RNA and enzyme levels suggested a possible transcriptional regulation of amylase in C. gigas as in vertebrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003600050003
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural changes in the uropygial gland of the male Japanese quail, Coturnix coturnix, after testosterone treatment (1986)
    Springer
    Cell & tissue research 246 (1986), S. 373-378 
    Details
    ISSN: 1432-0878
    Keywords: Uropygial gland ; Sebaceous gland ; Testosterone ; Japanese quail ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the uropygial gland of the male quail was compared to that of the sebaceous gland of the male rat after castration and testosterone treatment of both species. In intact animals, the differentiating cells of these glands displayed almost the same pattern as regards their smooth endoplasmic reticulum, an organelle involved in lipogenesis in both cases. Castration reduced the volume of this organelle, while testosterone administration restored cell morphology to a normal or supranormal level. Finally, this study showed that at ultrastructural level, there is a close functional analogy between the uropygial gland of quail and the sebaceous glands of rats as regards their androgen dependency. Consequently, the uropygial gland might be an attractive model for study of action of androgens on sebaceous-like glands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215900
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    Paper (German National Licenses)
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