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  • DNA hybridizations  (2)
  • CFU-GM  (1)
  • High energy shock waves  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The effect of High energy shock waves (HESW) on human bone marrow (1988)
    Springer
    Urological research 16 (1988), S. 427-429 
    Details
    ISSN: 1434-0879
    Keywords: HESW ; High energy shock waves ; CHO cells ; Chinese hamster ovary cells ; CFU-GM ; Colony forming units ; Granulocyte and macrophage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of High energy shock waves (HESW) on the viability and proliferation of normal human bone marrow cells was evaluated. A dose dependent increase in cytotoxicity with an increase in the number of HESW was demonstrated. In general 700 HESW immediately reduced the cell viability of bone marrow cells by 50%. The CFU-GM assay provides a method to evaluate the effect of HESW on the proliferative capacity of bone marrow. Following HESW treatment the colony forming ability of trypan blue excluding cells also felt in a dose dependent fashion, but some variation in sensitivity was noted. By comparing the sensitivity of various cells, the cells of normal human bone marrow were felt to be less sensitive to HESW effects than those of other tissue cultured cells or malignant cell lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00280023
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of the G-genome donor to Triticum timopheevii by DNA:DNA hybridizations (1985)
    Springer
    Biochemical genetics 23 (1985), S. 125-137 
    Details
    ISSN: 1573-4927
    Keywords: Triticum timopheevii ; Triticum speltoides ; G genome ; tetraploids ; evolution ; DNA hybridizations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In vitro DNA:DNA hybridizations and hydroxyapatite thermal-elution chromatography were employed to identify the diploid Triticum species ancestral to the G genome of Triticum timopheevii. Total genomic, unique-sequence, and repeated-sequence fractions of 3H-T. timopheevii DNA were hybridized to the corresponding fractions of unlabeled DNAs of T. searsii, T. speltoides, T. sharonensis, T. longissimum, and T. bicorne. The heteroduplex thermal stabilities indicated that, of the five species examined, T. speltoides was the most closely related to the G genome of T. timopheevii. Thus, T. spelotides appears to be the G-genome donor to T. timopheevii. The thermal stability profiles further indicated that the repeated DNA fractions from the five diploid species and the tetraploid T. timopheevii are more similar than the unique DNA fractions. This indicates that all of these species are closely related and that the sequences which comprise the current repeated fractions in the various species have not undergone any significant change since the formation of various species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499118
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Implication of Triticum searsii as the B-genome donor to wheat using DNA hybridizations (1983)
    Springer
    Biochemical genetics 21 (1983), S. 745-760 
    Details
    ISSN: 1573-4927
    Keywords: Triticum searsii ; B genome ; hexaploids ; origin ; wheats ; DNA hybridizations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In vitro DNA:DNA hybridizations and hydroxyapatite thermal chromatography were employed to help identify the species ancestral to the B genome of the polyploid wheats. We hybridized 3H-Triticum aestivum DNA to the unlabeled DNAs of T. urartu, T. speltoides, T. sharonensis, T. bicorne, T. longissimum, and T. searsii. 3H-Labeled DNA of T. urartu was hybridized with the DNA of a synthetic tetraploid, AADD. The heteroduplex thermal stabilities indicated that T. searsii was most closely related to T. aestivum (ABD) and that the genome of T. urartu was more closely related to the A genome than the B genome. The degree of reassociation which may have occurred between the six diploid species and the D genome of T. aestivum was evaluated by hybridizing 3H-T. tauschii DNA with the DNAs of the diploids. The results indicated that T. urartu had the least sequence homology to T. tauschii, the D-genome donor lending additional support to the conclusion that T. urartu is related to the A genome. Thus, it is highly probable that T. searsii is the B-genome donor to the polyploid wheats or a major chromosome donor if the B genome is, in fact, polyphyletic in origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00498921
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    Paper (German National Licenses)
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