ISSN:
0170-2041
Keywords:
Preparative-enzymatic synthesis
;
Cytidine 5′-triphosphate (CTP)
;
Cytidine 5′-monophosphosialate synthase
;
Cytidine 5′-monophosphosialate (CMP-Neu5Ac)
;
CTP regeneration, integrated
;
Chemistry
;
Organic Chemistry
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The preparative formation of cytidine 5′-triphosphate by an enzymatic process with adenylate kinase and pyruvate kinase is described. The enzymes may either be used free, in the “MEEC” technique, or immobilized on a synthetic copolymer of vinyl acetate and N,N′-divinylethyleneurea (“VA-Epoxy”). Cytidine 5′-monophosphosialate synthase (preferably isolated from calf brain) and inorganic pyrophosphatase were used to prepare the activated neuraminic acid cytidine 5′-monophosphosialate (CMP-Neu5Ac) from CTP and neuraminic acid in good yield. Finally, both processes could be integrated and led to an in situ approach which, owing to moderate yields, requires further development.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jlac.1990199001199
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