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  • 1
    Electronic Resource
    Electronic Resource
    Alterations in heart and serum lysosomal activities in streptozotocin-induced diabetes (1987)
    Springer
    Basic research in cardiology 82 (1987), S. 271-278 
    Details
    ISSN: 1435-1803
    Keywords: diabetes ; lysomal enzymes ; diabeticcardiomyopathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Alterations in the cardiac tissue and serum acid hydrolase activities were studied in chronic streptozotocin-induced diabetes in rats. No changes were observed in total cardiac tissue homogenate lysosomal enzyme activities at 4 weeks of diabetes but there were significant alterations in the distribution of selected enzymes. Significant decreases in nonsedimentable β-N-acetylglucosaminidase (NAG) and β-galactosidase (Gal) activities were observed at 4 weeks of diabetes. At 8 weeks of the disease, decreased activities of NAG and Gal were observed in heart homogenates but no changes were apparent in α-mannosidase (Man) or acid phosphatase activities. Nonsedimentable activities of NAG and both sedimentable and nonsedimentable activities of Gal were decreased at 8 weeks. At 16 weeks of the diabetic condition, increased activities of NAG, Gal and acid phosphatase were observed. This increase at 16 weeks of the disease was due to an increase in sedimentable enzyme activity. At all times of diabetes, serum enzyme activities were significantly increased. Insulin treatment reversed all of the observed changes in tissue homogenates, but serum levels were not completely reversed. These results suggest that cardiac lysosomal hydrolases are probably only involved in the later stages of the diabetic cardiomyopathy when extensive ultrastructural derangements are evident. The present evidence also suggests that the heart may be a source of serum hydrolase activities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01906859
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Alterations in heart sarcolemmal Ca2+-ATPase and Ca2+-binding activities due to oxygen free radicals (1990)
    Springer
    Basic research in cardiology 85 (1990), S. 45-54 
    Details
    ISSN: 1435-1803
    Keywords: freeradicals ; hydrogen peroxide ; Ca2+-ATPase ; Ca2+-binding ; heart sarcolemma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Effects of oxygen free radicals on Ca2+/Mg2+ ATPase and ATP-independent Ca2+-binding activities were examined in rat heart sarcolemma. Membranes were incubated with different oxygen radical generating media such as xanthine + xanthine oxidase, hydrogen peroxide, and hydrogen peroxide + Fe2+. In the presence of xanthine + xanthine oxidase, Ca2+ ATPase activity was stimulated and this effect was prevented by the addition of superoxide dismutase. Hydrogen peroxide also showed a significant increase in Ca2+-ATPase activity in a dose-dependent manner and this effect was blocked by catalase. On the other hand, a combination of hydrogen peroxide + Fe2+ decreased Ca2+-ATPase activity; this depression was prevented by the addition of D-mannitol. The observed change in Ca2+-ATPase activity due to oxygen free radicals was associated with changes in Vmax, whereas Ka remained unaffected. Both xanthine + xanthine oxidase and hydrogen peroxide increased whereas, hydrogen peroxide + Fe2+ inhibited the ATP-independent Ca2+-binding activities. It is suggested that oxygen free radicals may influence Ca2+ movements in the cell by altering the Ca2+/Mg2+ ATPase and Ca2+-binding activities of the membrane and these effects may be oxygen-radical species specific.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01907013
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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