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Digitale Medien

Expression of major bone extracellular matrix proteins during embryonic osteogenesis in rat mandibles (2000)

Sasano, Y. ; Zhu, Jing-Xu ; Kamakura, Shinji ; [weitere]

Springer

Anatomy and embryology 202 (2000), S. 31-37

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Details

ISSN: 1432-0568

Schlagwort(e): Key words Bone ; Calcification ; Type I collagen ; Noncollagenous proteins ; Immunohistochemistry

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract It is not known how bone proteins appear in the matrix before and after calcification during embryonic osteogenesis. The present study was designed to investigate expressions of the five major bone extracellular matrix proteins – i.e. type I collagen, osteonectin, osteopontin, bone sialoprotein and osteocalcin – during osteogenesis in rat embryonic mandibles immunohistochemically, and their involvement in calcification demonstrated by von Kossa staining. Wistar rat embryos 14 to 18 days post coitum were used. Osteogenesis was not seen in 14-day rat embryonic mandibles. Type I collagen was localized in the uncalcifed bone matrix in 15-day mandibles, where no other bone proteins showed immunoreactivity. Osteonectin, osteopontin, bone sialoprotein and osteocalcin appeared almost simultaneously in the calcified bone matrix of 16-day mandibles and accumulated continuously in 18-day mandibles. The present study suggested that type I collagen constitutes the basic framework of the bone matrix upon which the noncollagenous proteins are oriented to lead to calcification, whereas the noncollagenous proteins are deposited simultaneously by osteoblasts and are involved in calcification cooperatively.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/PL00008242

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Digitale Medien

Heat labile activating substance for ornithine decarboxylase activity in sea urchin eggs (1978)

Kusunoki, Shinichiro ; Yasumasu, Ikuo

New York, NY : Wiley-Blackwell

Gamete Research 1 (1978), S. 129-136

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Details

ISSN: 0148-7280

Schlagwort(e): sea urchin ; ornithine decarboxylase ; polyamine ; Ca2+ ; fertilization-induced activation ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie

Notizen: In sea urchin eggs, the activity of ornithine decarboxylase (ODC) [ E C 4.1.1.17] is detectable only in the particulate fraction yielded by centrifuging egg homogenates at 10,000g for 30 minutes. ODC activity in the particulate fraction isolated from fertilized eggs is higher than that from unferti-lized eggs. ODC activity in the particulate fraction isolated from either unfertilized or fertilized eggs is enhanced by adding the supernatant fraction obtained by centrifugation at 105,000g for two hours. Heating this supernatant at 70°C for 15 minutes results In complete loss of the stimulating capacity for ODC activity. Sea urchin eggs seem to contain heat labile activating substance(s) for ODC activity. The substance does not pass through the ultrafiltration membrane Diafro UM-10. Only eggs and unhatched embryos, in which mitosis occurs frequently, contain the activating substance. In the presence of the activating substance, Ca2+enhanced ODC activity.

Zusätzliches Material: 3 Ill.