ISSN:
1432-2013
Keywords:
MDCK-cells
;
Microelectrodes
;
Cell membrane potential
;
Potassium conductance
;
Sodium
;
Calcium
;
Barium
;
Amiloride
;
A23187
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract In incompletely confluent madin Darby canine kidney cells continuous measurements of the potential difference across the cell membrane (PD) were made with conventional microelectrodes during rapid changes of extracellular sodium and/or calcium concentration. During control conditions PD averages −50.6±0.7 mV. Reduction of extracellular sodium concentration from 131.8 to 17.8 mmol/l leads to a reversible hyperpolarization of the cell membrane to −65.3±1.1 mV. This hyperpolarization is not significantly reduced by omission of glucose or presence of amiloride (1 mmol/l) in the perfusates. Instead, 1 mmol/l amiloride depolarizes the cell membrane by +5.2±0.4 mV. 1 mmol/l barium depolarizes the cell membrane to −31.3±1.1 mV. Step increases of extracellular potassium concentration from 5.4 to 10 and 20 mmol/l depolarize the cell membrane by +5.5±0.5 mV and +16.5±1.8 mV respectively. In the presence of barium, the depolarizing effect of increasing extracellular potassium concentration and of amiloride is almost abolished. Reduction of extracellular sodium concentration in the presence of barium, however, leads to a transient hyperpolarization of the cell membrane. During this transient hyperpolarization, increasing extracellular potassium concentration depolarizes the cell membrane despite the continued presence of barium. Omission of extracellular calcium (EDTA) depolarizes the cell membrane by +36.7±3.2 mV. In the absence of extracellular calcium, the hyperpolarizing effect of reduced extracellular sodium concentration is markedly reduced (−4.5±1.2 mV). 2 μmol/l A23187 in the presence of extracellular calcium hyperpolarizes the cell membrane to −72.5±0.6 mV. In conclusion, reduction of extracellular sodium concentration increases the potassium conductance of the cell membrane, possibly by increasing intracellular calcium activity via an influence on the sodium/calcium-exchange.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00585300
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