ISSN:
1615-6102
Keywords:
Physcomitrella protonemata
;
Chloroplast division
;
Cytoskeleton
;
Cell division abnormalities
;
Microinjection
;
Moss mutant
;
Protoplasts
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary An X-ray induced mutant (PC22) of the moss,Physcomitrella patens was analysed with respect to its morphology, physiology and suitability for microculture techniques. The mutant protonemata are defective in bud formation and in chloroplast division. As a consequence of the latter, giant chloroplasts are formed which disturb the development of the phragmoplast, the formation of regular cross walls, and cell division. Abnormal cross walls are rich in callose. The actin cytoskeleton was found to be less regularly developed in the mutant than in the wild type. Three-dimensional analysis of the microtubular arrangement with confocal laser scan microscopy demonstrates a close association between spindle- or phragmoplast- and “interphase”-microtubules. The deficiencies in chloroplast division and in bud formation can partly be compensated for by exogeneously applied cytokinin. The suitability of this particular developmental mutant for further studies was shown by regeneration of protoplasts in microculture and microinjection of the fluorochrome Lucifer yellow into the chloroplast.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01354234
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