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RFLP mapping of a major bruchid resistance gene in mungbean (Vigna radiata, L. Wilczek) (1992)

Young, N. D. ; Kumar, L. ; Menancio-Hautea, D. ; [et al.]

Springer

Theoretical and applied genetics 84 (1992), S. 839-844

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ISSN: 1432-2242

Keywords: Callosobruchus ; DNA Markers ; Insect ; Legume ; Restriction fragment length polymorphisms

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Bruchids (genus Callosobruchus) are among the most destructive insect pests of mungbeans and other members of the genus, Vigna. Genetic resistance to bruchids was previously identified in a wild mungbean relative, TC1966. To analyze the underlying genetics, accelerate breeding, and provide a basis for map-based cloning of this gene, we have mapped the TC1966 bruchid resistance gene using restriction fragment length polymorphism (RFLP) markers. Fifty-eight F2 progeny from a cross between TC1966 and a susceptible mungbean cultivar were analyzed with 153 RFLP markers. Resistance mapped to a single locus on linkage group VIII, approximately 3.6 centimorgans from the nearest RFLP marker. Because the genome of mungbean is relatively small (estimated to be between 470 and 560 million base pairs), this RFLP marker may be suitable as a starting point for chromosome walking. Based on RFLP analysis, an individual was also identified in the F2 population that retained the bruchid resistance gene within a tightly linked double crossover. This individual will be valuable in developing resistant mungbean lines free of linkage drag.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00227394

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Identification of 2-hydroxy fatty acids in complex mixtures of fatty acid methyl esters by mass chromatographyAbbreviations: Hydroxy fatty acids are identified by h preceding chain length. (1974)

Laine, R. A. ; Young, N. D. ; Gerber, J. N. ; [et al.]

Chichester : Wiley-Blackwell

Biological Mass Spectrometry 1 (1974), S. 10-14

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ISSN: 1052-9306

Keywords: Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: Repetitive mass spectrometric scanning of gas-liquid chromatographic effuents at short (6 to 15 s) intervals, with a digital computer for data acquisition, reduction and display, allows plots (mass chromatograms) to be made of the intensities of specific ion fragments. Plotting of m/e values characteristic for certain classes of compounds and/or individual components reveals the location of constituents in the gas chromatogram. This technique was applied to the identification of trimethylsilyl derivatives of unsubstituted and 2-hydroxy fatty acid methyl esters in mixtures obtained from acid catalyzed methanolysis of cat brain galactocerebrosides. The plot of total ionization intensity vs scan number generated the gas-liquid chromatogram. Mass chromatograms of m/e 73 and [M - 59]+ gave the locations in the gas-liquid chromatogram of the hydroxy fatty acid derivatives, and mass chromatograms of m/e 74 and [M]+ located the unsubstituted fatty acid derivatives. Integrated ion intensities were used to determine the composition of the mixture of fatty acids.

Additional Material: 5 Ill.