ISSN:
1432-072X
Keywords:
Candida tropicalis
;
Utilization of n-alkane
;
Isolation of microbody
;
Catalase
;
Glyoxylate cycle
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Microbodies appearing abundantly in n-alkane-grown cells of Candida tropicalis pK 233 were isolated by means of sucrose density gradient centrifugation. Electron microscopical observation showed that the microbodies isolated were intact. Localization of catalase and d-amino acid oxidase in the isolated microbodies was confirmed. Isocitrate lyase, malate synthase and NADP-linked isocitrate dehydrogenase were also located in the microbody, but malate dehydrogenase, citrate synthase, aconitase and NAD-linked isocitrate dehydrogenase were not. Neither cytochrome P-450 nor NADPH-cytochrome c reductase, the components involved in the n-alkane hydroxylation system of the yeast, were detected in the microbody fraction.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00446647
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