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  • 1
    Electronic Resource
    Electronic Resource
    Regulation of ion channels in rat hepatocytes (1997)
    Springer
    Pflügers Archiv 435 (1997), S. 203-210 
    Details
    ISSN: 1432-2013
    Keywords: Key words Patch clamp ; Cell membrane potential ; K+ conductance ; K+ channels ; Cl ; channels ; cAMP ; Cell volume ; Progesterone ; Hepatocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Patch-clamp studies have been performed to elucidate single ion channels in rat hepatocytes. In rat hepatocytes two types of ion channel have been identified: an inwardly rectifying K+ channel with a mean inward conductance of 55 ± 6.5 pS (n = 20) and a mean outward conductance of 25 ± 3.2 pS (n = 20) in the inside-out configuration with 145 mmol/l KCl on either side of the patch as well as an outwardly rectifying Cl– channel with a mean outward conductance of 30 ± 4.5 pS (n = 8) and a mean inward conductance of 10 ± 2.3 pS (n = 6) in the inside-out configuration with symmetrical 145 mmol/l KCl. The open probability of these channels is virtually insensitive to Ca2+ activity on the intracellular side. Accordingly, the Ca2+ ionophore ionomycin had no effect on cell membrane potential. Dibutyryl-cAMP (db-cAMP) hyperpolarizes the cell membrane and increases the activity of the 55-pS inwardly rectifying K+ channel by reducing the duration of closure between bursts. Forskolin similarly hyperpolarizes the cell membrane. The inwardly rectifying K+ channel is inhibited by progesterone, while the outwardly rectifying Cl– channel is insensitive to progesterone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050502
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  • 2
    Electronic Resource
    Electronic Resource
    Exposure of perfused liver to hypotonic conditions modifies cellular nitrogen metabolism (1990)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 43 (1990), S. 355-361 
    Details
    ISSN: 0730-2312
    Keywords: cell volume ; glutamine ; ammonia ; urea synthesis ; metabolic regulation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Isolated livers were exposed to hypotonic perfusates. As shown previously, this hypotonic challenge leads to initial cell swelling, followed by volume regulatory ion fluxes, largely restoring cell volume within approximately 6 min. However, the hepatocyte is left in an altered metabolic state, which is characterized by marked stimulation of hepatic glutamine uptake and degradation and transient release of glutamate from the liver. Urea formation from glutamine and alanine is stimulated, whereas hepatic ammonia uptake and utilization for urea and glutamine synthesis decreases. These observations reveal a hitherto unrecognized factor modulating hepatic function during intestinal absorption.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240430407
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    Paper (German National Licenses)
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