ISSN:
0730-2312
Keywords:
breast cancer cell line
;
CAMA-1
;
Intron Differential RNA/PCR
;
gene expression
;
EGF receptor
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
To elucidate the relationship between epidermal growth factor (EGF)/transforming growth factor (TGF-α) and estradiol-17β (E) in cell proliferation, we examined their effects on the breast cancer cell line, CAMA-1. While E was able to consistently induce cell proliferation under a variety of experimental conditions, EGF/TGF-α was without effect. Despite the presence of the receptor (EGFR) gene, mature EGFR protein and mRNA were not detected by radioreceptor assay, 35S Met-labelling, and the Intron Differential RNA/PCR method under conditions in which cells remain responsive to E. Furthermore, TGF-α is not an autocrine factor in CAMA-1 cells. We demonstrated unequivocally that EGF/TGF-α interaction with EGFR is not an obligatory event in mediating estrogen-stimulated cell proliferation.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcb.240460206
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