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  • Cell & Developmental Biology  (37)
  • Electroretinogram  (2)
  • smooth muscle  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Photoaffinity labeling of the DDT1 MF-2 cell α1-adrenergic receptor (1985)
    Springer
    Molecular and cellular biochemistry 67 (1985), S. 47-53 
    Details
    ISSN: 1573-4919
    Keywords: α1-adrenergic receptor ; membrane ; photoaffinity ; smooth muscle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary In this study, we have used an α1-adrenergic receptor photoaffinity ligand, 2-[4-(4-azido-3-iodo-benzoyl)-piperazin-1-yl]-4-amino-6, 7-dimethoxyquinazoline (125I-APD), to label covalently the α1-adrenergic receptor in a smooth muscle cell line. Our results indicate that in the absence of light, (125I)APD binds reversibly to a site in the DDT1 MF-2 cell membranes having pharmacological characteristics of an α1-adrenergic receptor. Following incorporation of (125I)ADP into partially purified membranes a single labeled band of protein with a Mr of 81 000 was visualized by autoradiography following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Incorporation of (125I)-APD into this band was affected by adrenergic agonists and antagonists in a manner consistent with an α1-adrenergic interaction. Prazosin (α1-selective) blocked incorporation of the label into the Mr = 81 000 protein while yohimbine (α2-selective) did not. Of the adrenergic agonists, (−)-epinephrine and (−)-norepinephrine but not (−)-isoproterenol blocked labeling of the Mr − 81 000 protein. We conclude that the ligand binding site of the DDT1 MF-2 cell α1-adrenergic receptor resides in a Mr = 81 000 protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220985
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  • 2
    Electronic Resource
    Electronic Resource
    Glucocorticoid induction of β-adrenergic receptors in the DDT1 MF-2 smooth muscle cell line involves synthesis of new receptor (1987)
    Springer
    Molecular and cellular biochemistry 74 (1987), S. 21-27 
    Details
    ISSN: 1573-4919
    Keywords: glucocorticoid receptor ; β-adrenergic receptor regulation ; smooth muscle ; adenylate cyclase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract We have shown that glucocorticoids induce the appearance of β2-adrenergic receptors in membranes of the ductus deferens smooth muscle cell line (DDT1 MF-2). A concomitant increase in isoproterenol stimulated adenylate cyclase activity in the absence of exogenously applied GTP was observed as was a significantly increased (p 〈 0.05) sensitivity of the adenylate cyclase system to exogenously applied GTP. However, no significant difference in the maximal velocity of adenylate cyclase between control and steroid treatment was measurable in the presence of sodium fluoride. Induction of β2-adrenergic receptors in DDT1 MF-2 cells is correlated with the presence of steroid receptors (androgen and glucocorticoid) in the cells since estrogens and progesterones had no effect on receptor levels. Finally, utilizing dense amino acid labeling of cells to measure old versus newly synthesized receptor sites by a density shift method, we have documented that glucocorticoid induction of β2-adrenergic receptors involves synthesis of new receptor protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221909
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  • 3
    Electronic Resource
    Electronic Resource
    Fundus pigmentation and the dark-adapted electroretinogram (1992)
    Springer
    Documenta ophthalmologica 80 (1992), S. 1-11 
    Details
    ISSN: 1573-2622
    Keywords: Electroretinogram ; fundus pigmentation ; luminance-response function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Dark-adapted electroretinograms were obtained over a 3.6-log range of stimulus intensities from 17 black and 15 white normal subjects. Subjects were grouped on the basis of light or dark fundus pigmentation, determined from digitized fundus photographs. B-wave amplitudes for each group were fitted by the Naka-Rushton equation, and the measures Vmax, log K, and n were determined. The luminance-response functions revealed that subjects with light fundi had larger b-wave amplitudes at all luminance levels. There was a significant difference between groups for Vmax and n but not for log K. A comparison of b-wave implicit times showed no significant difference between subjects with dark and light fundi. Ancillary tests and multiple regression analysis suggested that the relationship between Vmax and fundus pigmentation could not be attributed to age, gender, refractive error, axial length or intraocular pressure. The results have implications for the collection of normative electroretinographic data and for the interpretation of electroretinogram results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00161226
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  • 4
    Electronic Resource
    Electronic Resource
    Fundus pigmentation and the electroretinographic luminance-response function (1993)
    Springer
    Documenta ophthalmologica 84 (1993), S. 61-69 
    Details
    ISSN: 1573-2622
    Keywords: Electroretinogram ; Fundus pigmentation ; Luminance-response function ; Photopic hill ; Second limb
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Dark-adapted and light-adapted electroretinogaphic luninance-response functions were recorded from subjects with light or dark fundus pigmentation based on digitized fundus photographs. For dark- and light-adapted electroretinograms, subjects with dark fundi had smaller b-wave amplitudes at all luminance levels. There was no significant difference in b-wave implicit time for the dark-adapted electroretinogram, but there was a significant difference for the light-adapted ERG between the two groups. The results suggest that fundus pigmentation should be considered in the interpretation of electroretinogram results. A possible mechanism for the influence of fundus pigmentation on b-wave amplitude is based on increased resistance associated with melanin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01203283
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  • 5
    Electronic Resource
    Electronic Resource
    Facial morphology and vibrissal movement in the golden hamster (1985)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 183 (1985), S. 199-217 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The major cranial vibrissae in the golden hamster can be moved in complex ways that suggest they are served by a finely controlled motor system. Movements are hypothesized to be the products of (1) differential blood flow and pressure regulation in the sinus surrounding each vibrissal follicle, (2) contractions of the striated facial muscles, and (3) elastic rebound in the connective tissues. The vasculature contributes hydrostatic forces that (a) erect the vibrissae slightly and distort their connective tissue bedding, (b) rigidify the vibrissal capsules, thus forming firm bases of attachment for certain facial muscles, and (c) theoretically provide a pressure plate around the follicle, important in lowering the firing thresholds of receptor endings. The facial muscles supply the major forces in erection and protraction of the vibrissae by acting on both the capsules and the connective tissue bedding. The connective tissues are organized into capsular and extracapsular systems that serve to stabilize the vibrissae and return them to initial rest positions.The slight movements of the genal vibrissa are the effects of vascular and connective tissue dynamics, the musculature being uninvolved. Wide angle movements of the supraorbital vibrissae are products of the vasculature and connective tissues, plus contractions of the Mm. orbicularis oculi and frontalis. Mystacial vibrissal movement is quite complex. The vasculature supplies a small degree of capsular erection and mystacial pad distortion, but primarily rigidifies the capsules. The bulk of erection and protraction is produced by the M. nasolabialis profundus (NLP) and the vibrissal capsular muscles (VCM). The NLP distorts the mystacial pad; the VCM tilt the capsules relative to the pad. Retraction is mainly accomplished by elastic rebound in the pad, this being aided in its extreme degrees by the Mm. nasolabialis and maxillolabialis. The Mm. nasolabialis superficialis and buccinator pars orbicularis oris help to spread the vibrissae into a dorsoventral fan and stabilize the mystacial pad during whisking.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051830208
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  • 6
    Electronic Resource
    Electronic Resource
    Inhibition of heat shock (stress) protein induction by deuterium oxide and glycerol: Additional support for the abnormal protein hypothesis of induction (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 139 (1989), S. 219-228 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The patterns of radioactively labeled proteins from cultured chicken embryo cells stressed in the presence of either D2O or glycerol were analyzed by using one-dimensional polyacrylamide gel electrophoresis. These hyperthermic protectors blocked the induction of stress proteins during a 1-hour heat shock at 44°C. The inhibitory effect of glycerol but not D2O on the induction of heat shock proteins could be overcome by increased temperature. By using transcriptional run-on assays of isolated nuclei and cDNA probes to detect hsp70- and hsp88-specific RNA transcripts, it was shown that the D2O and glycerol blocks occurred at or before transcriptional activation of the hsp70 and hsp88 genes. After heat-stressed cells were returned to 37°C and the protectors were removed, heat shock proteins were inducible by a second heating. This result and the fact that the chemical stressor sodium arsenite induced stress proteins in glycerol medium indicated that the treatments did not irreversibly inhibit the induction pathways and that the stress response could be triggered even in the presence of glycerol by a stressor other than heat. In principle then, cells incurring thermal damage during a 1-hour heat shock at 44°C in D2O or glycerol medium should be competent to respond by inducing heat shock proteins during a subsequent recovery period at 37°C in normal medium. We found that heat shock proteins were not induced in recovering cells, suggesting that glycerol and D2O protected heat-sensitive targets from thermal damage. Evidence that the heat-sensitive target(s) is likely to be a protein(s) is summarized. During heat shocks of up to 3 hours duration, neither D2O nor glycerol significantly altered hsp23 gene activity, a constitutively expressed chicken heat shock gene whose RNA transcripts and protein products are induced by stabilization (increased half-life). During a 2-hour heat shock, glycerol treatment blocked the heat-induced stabilization of hsp23 RNA and proteins; however, D2O treatment only blocked RNA transcript stabilization, effectively uncoupling the hsp23 protein stabilization pathway from hsp23 RNA stabilization and transcriptional activation of hsp70 and hsp88 genes.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041390202
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  • 7
    Electronic Resource
    Electronic Resource
    Volumetric compartmentalization of the cranial cerebrospinal fluid system determined radiographically in the catSupported by N.I.H. grant NB-04456.Presented as a preliminary report at the April, 1965 meeting of the Federation of American Societies for Experimental Biology (Federation Proc., 24: 399). (1966)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 155 (1966), S. 305-313 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A volume-distribution analysis of the water soluble contrast medium, meglumine iothalamate, injected into various ventricular and subarachnoid sites was accomplished radiographically in the cat with the aid of a newly designed screw-type cannula having a deadspace of 6 to 8 m̈l. The cannula is positioned stereotaxically and mounts directly and permanently in the cranium by a single self-tapping insertion maneuver. As little as 100 m̈l of solution injected into the anterior horn of the lateral ventricle, or 50 and 20 m̈l into the third and fourth ventricles, respectively, was visualized immediately in the lateral apertures of the fourth ventricle connecting with the subarachnoid spaces. Injection of 50 m̈l into the posterior horn of the lateral ventricle delineated the entire ventricle as well as the interventricular foramen. A volume of 12 m̈l deposited in the base of the third ventricle served to define the hypothalamic cleft and infundibular recess. If rate of injection was not excessive, the solution could be introduced into the third and fourth ventricles without penetrating into the more anterior regions. Radiopaque medium injected into subarachnoid spaces (cisternae ambiens and cerebellomedullaris) did not enter the ventricular system.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091550304
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  • 8
    Electronic Resource
    Electronic Resource
    Daily rhythmic variations in blood coagulation times in ratsThis work was supported by Research grant no 4659 from U.S.P.H.S., N.I.H. (1967)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 157 (1967), S. 657-665 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: With all other environmental factors rigidly standardized, normal Sprague-Dawley rats were maintained under the following schedules: (1) 12 hours of artificial light 0600 to 1800 alternating with 12 hours of darkness-LD; (2) reversal of the first-DL; (3) constant darkness-DD, and (4) constant illumination-LL.After the animals had been under a specific lighting regimen for at least three weeks, blood coagulation times were determined on separate groups of 8 to 16 animals at bi-hourly intervals during a 24-hour period. Significant daily fluctuations or rhythms in coagulation times under all lighting conditions were found by plotting each of the bi-hourly mean values as a function of time. The temporal phasing of all rhythms in LD, DL, LL and DD were similar. The major changes found in the different lighting schedules involved overall magnitude, e.g., the overall 24-hour mean coagulation time for rats maintained in LL was 21% greater than for rats maintained in DD. Although total adrenalectomy or adrenal medullectomy did not abolish the characteristic LD rhythm, there was about a 100% decrease in the mean 24-hour coagulation time of LD adrenalectomized animals when compared to normal LD animals. No significant decrease was seen in adrenal demedullectomized animals when similarly compared with normal LD animals.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091570411
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  • 9
    Electronic Resource
    Electronic Resource
    Circadian variation in cell division of the mouse alimentary tract, bone marrow and corneal epithelium (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 191 (1978), S. 479-486 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Circadian rhythms in DNA synthesis are described for the tongue epithelium, five different regions of the alimentary canal (gut) -esophagus, stomach, duodenum, jejunum and rectum- and bone marrow in a group of BDF1 male mice. A circadian rhythm is also described for the mitotic index in the corneal epithelium in the same mice. The data document for the first time in the same animals the dramatic variation in cell division encountered from one region of the gut to another. This variation is seen in the amplitudes of the rhythms as well as in the over-all 24-hour means. On the contrary, the phasings of the rhythms in the different regions of the gut are remarkably similar. In this study, where the mice were standardized to 12 hours of light (0600-1800) alternating with 12 hours of darkness, the peak of the DNA-synthesis rhythm occurred around the time of transition from dark to light, and the trough around the time of transition from light to dark. The implications of these findings, and those of others, to the study of cell kinetics and to cancer chemotherapy are discussed.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091910407
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  • 10
    Electronic Resource
    Electronic Resource
    Mitotic activity in the human epidermis (1959)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 135 (1959), S. 7-19 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091350103
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