ISSN:
0021-9541
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
The murine myelomonocytic leukemia WEHI-3B exists as differentiation-inducible (D+) and noninducible (D-) cell lines. Both lines produce a CSF species that stimulates exclusively the formation of neutrophil granulocyte colonies. This G-CSF copurifies with a mast cell growth factor but can be separated from M- and GM-CSF. NZB bone marrow is unresponsive to G-CSF stimulation. WEHI-3B D+ cells can be induced to terminal granulocyte differentiation by a factor present in murine and human postendotoxin serum that is different from G-CSF present in WEHI-3B D+ or D- CM since the latter has little or no leukemia differentiation-inducing activity. Endotoxin treatment of C. parvum primed mice leads to simultaneous induction of serum activities with selective action on myeloid leukemic cells, a serum differentiation inducing activity and a leukemic colony inhibitory activity. These factors act synergistically to block leukemic stem cell self-renewal. The results suggest that a variety of inducible factors may have potent and selective antileukemic activity.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1041130411
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