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  • 1
    Electronic Resource
    Electronic Resource
    Entry criteria for extracorporeal membrane oxygenation In neonates with congenital diaphragmatic hernia treated with high-frequency oscillatory ventilation (1996)
    Springer
    Pediatric surgery international 11 (1996), S. 532-535 
    Details
    ISSN: 1437-9813
    Keywords: Extracorporeal membrane oxygenation ; High-frequency oscillatory ventilation ; Congenital diaphragmatic hernia ; Oxygenation index ; Alveolararterial oxygen gradient
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Although respiratory management with high-frequency oscillatory ventilation (HFOV) has generally been used for neonates with congenital diaphragmatic hernia (CDH), entry criteria for extracorporeal membrane oxygenation (ECMO) based on data from patients who underwent HFOV have not yet been reported. To establish entry criteria for ECMO in such patients, we retrospectively studied 36 neonates with CDH treated by HFOV in our institutions between 1986 and 1994. From the admission records, preductal and postductal arterial blood gas data and HFOV ventilation conditions for 72 h after admission were extracted. Oxygenation index (01) and alveolar-arterial oxygen gradient (A-aD02) time interval combinations were calculated. Patients were divided into two groups: candidates for ECMO (n = 22) who underwent ECMO (n = 18) or died without ECMO (n = 4); and non-candidates (n = 14), who survived without ECMO. Blood gas data in patients placed on ECMO were comparable to those in patients who died without ECMO: mean pre- and postductal OI for 4 h 〉 30, postductal A-aD02 ≥620 mmHg for 4 h, postductal A-aD02 ≥580 mmHg for 8 h, and postductal A-aD02 ?550 mmHg for 12 h showed better sensitivity with a specificity of more than 90% compared to entry criteria that had previously been used in our institutions: a postductal OI 〉40 for 4 h and postductal A-aDO2 ≥610 mmHg for 8 h. In addition, a combination of preand postductal OI 〉30 for 4 h indicated a sensitivity of 95.5% and a specificity of 92.9%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00626059
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  • 2
    Electronic Resource
    Electronic Resource
    Prenatal diagnosis of congenital diaphragmatic hernia and pulmonary hypoplasia and therapeutic strategy (1996)
    Springer
    Pediatric surgery international 11 (1996), S. 512-517 
    Details
    ISSN: 1437-9813
    Keywords: Prenatal diagnosis ; Congenital diaphragmatic hernia ; Pulmonary hypoplasia ; High-frequency oscillatory ventilation ; Extracorporeal membrane oxygenation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The outcome of fetuses with congenital diaphragmatic hernia (CDH) has been reported to be fatal when pulmonary hypoplasia (PH) is severe. As an indicator of PH, we attempted to measure the lung-thorax transverse area ratio (L/T) using ultrasonic echography. Immediate postnatal surgery was performed using high-frequency oscillatory ventilation (HFOV) and sometimes followed by extracorporeal membrane oxygenation (ECMO). Eighteen fetuses were treated and 14 survived. L/T correlated well with the best preductal arterial blood gas data before surgical reduction during manual ventilation and HFOV, while preductal PO2 and alveolar-arterial oxygen differences from patients managed with HFOV were better than those in patients with manual ventilation. Although L/T also correlated with the duration of O2 therapy and hospitalization in survivors without major anomalies, there was no significant difference between L/T in survivors and nonsurvivors. Because delayed institution of ECMO and complications related to ECMO management seemed to be a major cause of death in non-survivors, the unsalvageable L/T due to PH was estimated to be below 0.06 for HFOV and below 0.1 for conventional ventilation based on the correlation between L/T and preductal P02. These results suggest that L/T is a useful indicator of PH in patients with CDH and also that HFOV is advantageous in treating CDH with PH. The advantage of prenatal diagnosis in predicting unsalvageable L/Ts, should be considered in the therapeutic strategy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00626055
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  • 3
    Electronic Resource
    Electronic Resource
    Organelle DNA polymorphism in apple cultivars and rootstocks (1992)
    Springer
    Theoretical and applied genetics 83 (1992), S. 963-967 
    Details
    ISSN: 1432-2242
    Keywords: Apple ; Chloroplast DNA ; Mitochondrial DNA ; RFLP ; Cytoplasmic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphisms (RFLPs) have been used to detect chloroplast (cp) and mitochondrial (mt) DNA variation among 18 apple cultivars and three rootstocks. The distribution of RFLP patterns allowed the assignment of these genotypes into three groups of cytoplasmic relatedness. Our results also demonstrate maternal inheritance of cp- and mtDNAs in apple. Thus, the organelle DNA assay provides a convenient and reliable method to assess cytoplasmic diversity within the apple germ-plasm collection and to trace the maternal lineages involved in the evolution of apple.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232957
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Cloning and expression of two autolysin genes, cwlU and cwlV, which are tandemly arranged on the chromosome of Bacillus polymyxa var. colistinus (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 738-748 
    Details
    ISSN: 1617-4623
    Keywords: Key words Peptidoglycan ; Cell wall hydrolase gene ; N-acetylmuramoyl-L-alanine amidase ; Bacillus polymyxa var. colistinus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cwlV gene, which encodes Bacillus polymyxa var. colistinus autolysin was cloned and sequenced. cwlV comprises a 1497-bp ORF and encodes a polypeptide of 499 amino acid (aa) residues (Mr of 53,707 Da). The N-terminal sequence of the mature 23-kDa CwlV protein is NSXGKKVVVIDAGXGAKD(X, undetermined aa); this processed form corresponds to the C-terminal portion (183 aa, Mr of 20,050 Da) of the cwlV ORF. Sequencing of the flanking region revealed that another putative autolysin gene, cwlU, is located upstream of cwlV. cwlU encodes a polypeptide of 524 aa and its deduced sequence is 34.9% identical to the full-length sequence of CwlV. Downstream of cwlV, the genes for a deduced lipoprotein (OrfW), an endonuclease III homolog (Nth), a non-homologous OrfX, a glutathione peroxidase homolog (Gpx), and the N-terminal region of OrfZ containing a ATP/GTP-binding site motif were found. Northern blotting and primer-extension analyses revealed that cwlU is transcribed as a single cistron, but cwlV is transcribed with orfW. The unprocessed forms of CwlV and CwlU (VΔS and UΔS, respectively) and their predicted mature forms (Vcat and Ucat, respectively) were expressed in, and purified from, Escherichia coli. Enzyme analysis indicated that VΔS and Vcat exhibit low and high cell wall hydrolase activities toward B. polymyxa cell wall, respectively, but UΔS and Ucat exhibit almost no and low cell wall hydrolase activities, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051136
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