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  • 1
    Electronic Resource
    Electronic Resource
    Modulation of granulocyte oxidative response by recombinant interferon α2 and γ (1986)
    Springer
    Archives of dermatological research 278 (1986), S. 274-276 
    Details
    ISSN: 1432-069X
    Keywords: Interferon α2 ; Interferon γ ; Granulocytes ; Chemiluminescence ; Lucigenin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Interferon (IFN) has been described to influence various cellular functions. In this study we investigated whether the oxidative response of polymorphonuclear leukocytes (PMN) is also affected by IFN. In order to exclude the possible influence of impurities in IFN preparations, only recombinant human IFN α2 or γ were used. Lucigenin-dependent chemiluminescence (CL) of PMN was measured to assess the production of oxygen radicals. IFN γ at a concentration of more than 10 ng/ml elicited a minimal CL response in PMN. When PMN were incubated with IFN γ for 1 h and then stimulated with chemotactic peptide f-met-phe (FMP), zymosan-activated serum (ZAS), zymosan particles, or phorbol-myristate acetate (PMA), the CL response was increased as consequence of the generally enhanced oxidative metabolism. IFN α2 showed no such effect at any concentration tested. A 5-min pretreatment with IFN γ decreased the ZAS response but did not affect the reaction to the other stimuli. The possibility of a generation of IFN by PMN during the assay could be excluded as no IFN activity could be detected in an antiviral assay after stimulation of PMN for 6 h with PolyIxPolyC, LPS, ConA, C. parvum, PMA, zymosan, or FMP. The modulation of granulocyte activity by IFN γ may be important in the regulation of the anti-inflammatory response of PMN.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407737
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Inhibitory effect of sulfonated shale oils (ammonium bituminosulfonate) on the stimulation of neutrophilic granulocytes by the chemotactic tripeptide f-Met-Leu-Phe (1986)
    Springer
    Archives of dermatological research 278 (1986), S. 190-193 
    Details
    ISSN: 1432-069X
    Keywords: Adherence ; Bituminosulfonate ; Chemiluminescence ; Chemotactic factor ; Enzyme release ; Neutrophils ; Superoxide anion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Sulfonated shale oils (ammonium bituminosulfonate, ichthammol, Ichthyol), shown previously to induce the directed migration of human neutrophils in Boyden chambers and to inhibit the directed migration towards the chemotactic factors C5a, LTB4, and f-Met-Leu-Phe, were studied for their effect on other neutrophil functions, which are stimulated by chemotactic factors. Like other chemotactic factors ammonium bituminosulfonate increased the adherence of neutrophils to nylon fibers, but it did not induce the release of the primary granule enzyme glucosaminidase from cytochalasin B-treated cells and it did not stimulate the production of oxygen radicals as measured by lucigenin-dependent chemiluminescence if studied under nontoxic conditions. When added together with the chemotactic tripeptide f-Met-Leu-Phe, ammonium bituminosulfonate inhibited adherence augmentation, enzyme release, and oxygen-radical production induced by the chemotactic factor. The results indicate that ammonium bituminosulfonate not only inhibited chemotactic migration but the whole spectrum of neutrophil functions induced by a chemotactic factor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412922
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Detection of complement activation in human serum using C5S-Induced chemiluminescence of human granulocytes (1985)
    Springer
    Archives of dermatological research 278 (1985), S. 41-43 
    Details
    ISSN: 1432-069X
    Keywords: Complement ; Anaphylatoxins ; C5a ; Chemiluminescence ; Granulocytes ; Lucigenin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The complement system can be activated by many factors, including immune complexes, leading to the generation of biologically active split products like C5a anaphylatoxin. This study presents a technique which may be used for measuring C5a activity in human serum. The tetanus-anti-tetanus immune complex and aggregated human IgG were used as model activators of the complement cascade. The C5a activity was measured by C5a-induced chemiluminescence of granulocytes; furthermore, a radioimmunoassay was used to detect the C5a peptide. There was a strongly positive correlation between the two assay systems. The described method should be useful as an alternative means of detecting complement activation in the serum of patients with inflammatory diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412494
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of tumour necrosis factor alpha in vivo on human granulocyte oxidative metabolism (1991)
    Springer
    Archives of dermatological research 283 (1991), S. 362-365 
    Details
    ISSN: 1432-069X
    Keywords: TNFα ; PMN ; Oxidative metabolism ; Chemiluminescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Tumour necrosis factor alpha (TNFα) effectively stimulates the oxidative metabolism of human PMN in vitro. Moreover, preincubation of PMN with TNFα has been shown to result in an altered response of the target cells to subsequent stimulation. In the present study the response of PMN to stimulation in vitro was investigated in patients with metastasizing malignant melanoma receiving bolus injections of recombinant human TNFα as therapy. TNFα was given daily for 5 days. Blood samples were taken prior to TNFα administration on days 1 to 4 and on day 8. Lucigenin-enhanced chemiluminescence (CL) was used as a sensitive measure of granulocyte oxidative metabolism. PMN were stimulated with TNFα, TNFΒ, GM-CSF, PMA, opsonized zymosan and f-met-leu-phe. A significant increase in CL responses was detected upon stimulation with TNFα, TNFΒ and PMA from day 1 to day 3, whereas no significant changes were observed for the background activity or when GM-CSF or opsonized zymosan were used as stimuli. On day 4 all CL responses returned to the day 1 starting level. A further significant decrease was observed on day 8 upon stimulation with TNFα, TNFΒ and GM-CSF. In contrast, the effect induced by f-met-leu-phe reached a maximum on day 4, but the CL response was found to be at the starting level on day 8. The results indicate that TNFα induces significant changes in PMN response to distinct stimuli in vivo. Moreover, it may be possible that continous daily infusions with TNFα induce a hyposensitization of PMN oxidative metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00371816
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    Paper (German National Licenses)
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