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  • Chemistry  (4)
  • Glycolysis  (2)
  • Distribution  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Purification and characterization of an ADP-dependent phosphofructokinase from Thermococcus zilligii (1999)
    Springer
    Extremophiles 3 (1999), S. 121-129 
    Details
    ISSN: 1433-4909
    Keywords: Key words Thermococcus ; Pyrococcus ; Thermophilic ; Phosphofructokinase ; Evolution ; ADP ; Glycolysis ; ATP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ADP-dependent phosphofructokinase (PFK) from Thermococcus zilligii has been purified 950 fold; it had a specific activity of 190 U mg−1. The enzyme required Mg2+ ions for optimal activity and was specific for ADP. The forward reaction kinetics were hyperbolic for both cosubstrates (pH optimum of 6.4), and the apparent K m values for ADP and fructose-6-phosphate were 0.6 mM (apparent V max of 243 U mg−1) and 1.47 mM (apparent V max of 197 U mg−1), respectively. Significantly, the enzyme is indicated to be nonallosteric but was slightly activated by some monovalent cations including Na+ and K+. The protein had a subunit size of 42.2 kDa and an estimated native molecular weight of 66 kDa (gel filtration). Maximal reaction rates for the reverse reaction were attained at pH 7.5–8.0, and the apparent K m values for fructose-1,6-bisphosphate and AMP were 0.56 mM (apparent V max of 2.9 U mg−1) and 12.5 mM, respectively. The biochemical characteristics of this unique ADP-dependent enzymatic activity are compared to ATP and pyrophosphate-dependent phosphofructokinases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007920050107
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation of an extremely thermophilic chemoorganotrophic anaerobe similar to Dictyoglomus thermophilum from new zealand hot springs (1987)
    Springer
    Archives of microbiology 147 (1987), S. 21-24 
    Details
    ISSN: 1432-072X
    Keywords: Dictyoglomus ; Fervidothrix ; Description ; Rotund bodies ; Extremely thermophilic ; Obligate anaerobe ; Fermentation products ; Physiology ; Distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A strain similar to Dictyoglomus thermophilum, isolated from a New Zealand hot spring, is described. This strictly anaerobic, Gram-negative, non-motile and nonsporulating bacterium usually exists as long thin filaments of 5 to 25 μm by 0.35 to 0.45 μm. Rotund bodies are commonly observed. Thin sections of the cells revealed a two-layered cell wall. The optimum temperature and pH for growth was 70°C and 7.0 and 7.5 respectively. No growth was observed at 40°C and 85°C or at pH 4.5 to pH 9.0. The organism fermented glucose, maltose, mannose, xylose, lactose, cellobiose, galactose and sucrose and produced acetate as the major end-product with significant amounts of lactate, H2 and CO2 and only traces of ethanol. The doubling time on glucose was 10 h. The DNA base composition was 29.5% guanine plus cytosine as determined by the thermal denaturation method. Growth was inhibited by penicillin, tetracycline and chloramphenicol indicating that the organism was a eubacterium. These features are in common with the newly described species Dictyoglomus thermophilum to which the New Zealand isolate belongs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492899
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  • 3
    Electronic Resource
    Electronic Resource
    Phosphofructokinase activities within the order Spirochaetales and the characterisation of the pyrophosphate-dependent phosphofructokinase from Spirochaeta thermophila (1999)
    Springer
    Archives of microbiology 172 (1999), S. 401-406 
    Details
    ISSN: 1432-072X
    Keywords: Key words Spirochetes ; Phosphofructokinase ; Treponema ; Leptospira ; Spirochaeta ; Glycolysis ; ¶Pyrophosphate ; Thermophile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The subtype of phosphofructokinase activity, either ATP-, ADP- or pyrophosphate-dependent, present in members of three genera from the Spirochaetales was investigated. The individual species/strains examined included Spirochaeta alkalica, S. asiatica, S. halophila, S. isovalerica, S. litoralis, S. zuelzerae, S. thermophila, two thermophilic spirochetes, Treponema bryantii, T. denticola, ¶T. pectinovorum, Leptospira biflexa and L. interrogans. All of the Spirochaeta strains, regardless of their phenotype, possessed primarily a pyrophosphate-dependent phosphofructokinase. In contrast, T. bryantii, T. denticola and L. biflexa had predominantly an ATP-dependent activity, whereas no activity was detected in T. pectinovorum or ¶L. interrogans. The results suggest that pyrophosphate-dependent phosphofructokinase activity may be a reliable phenotypic marker for the genus Spirochaeta and that there are potentially interesting differences in how the catabolism of saccharides is controlled among members of genera within the Spirochaetales. The pyrophosphate-dependent phosphofructokinase from S. thermophila strain RI 19.B1 was purified (303-fold) to homogeneity and biochemically characterised. The S. thermophila enzyme displayed hyperbolic kinetics with respect to both the forward and reverse cosubstrates and was not significantly affected by traditional activators or inhibitors of phosphofructokinase. The biochemical characterisation represents the first spirochete phosphofructokinase to be described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050777
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  • 4
    Electronic Resource
    Electronic Resource
    Polymerization of avidin and streptavidin with aromatic bisbiotin ligands (1994)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 32 (1994), S. 1331-1340 
    Details
    ISSN: 0887-624X
    Keywords: affinity polymerization ; aromatic bisbiotin ligands ; avidin ; biopolymers ; molecular recognition ; streptavidin ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The synthesis of a range of aromatic bisbiotin ligands is described and their ability to polymerize the proteins avidin and streptavidin is investigated. The formation of protein polymers is confirmed by gel chromatography and transmission electron microscopy. It is shown that the degree of polymerization depends not only on the length of the ligand but also on the stereochemistry of the group linking the distal biotin moieties. © 1994 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1994.080320714
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  • 5
    Electronic Resource
    Electronic Resource
    The degradation of lignocellulosics by extremely thermophilic microorganisms (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 33 (1989), S. 1495-1499 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Five extremely thermophilic cellulose-degrading isolates obtained from New Zealand thermal springs were tested for their ability to degrade a number of natural lignocellulosic substrates. Degradation by three of the isolates was generally similar to that by the moderate thermophile, Clostridium thermocellum but occurred at a higher temperature. The New Zealand isolates were also found to grow on xylan as sole carbohydrate source, which probably extends their attack to hemicellulose.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260331118
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  • 6
    Electronic Resource
    Electronic Resource
    Some properties of a β-galactosidase from an extremely thermophilic bacterium (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 1141-1145 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An inducible β-galactosidase from an extremely thermophilic organism, Thermus strain 4-1A, has been isolated and partially purified. There were significant dissimilarities to T. aquaticus β-galactosidase. It had a pl of 4.5, was inhibited by sulphydryl inhibitors and a number of transition metals, and was activated by EDTA and SH-containing reagents. The β-galactosidase showed strong product inhibition, and weaker inhibition by some other mono- and disaccharides. It was very stable up to 90°C at pH 8. On immobilization by diazonium linkage to porous glass, the pH optimum (6.0), the KM with ONPG (5mM) and the product inhibition were not altered.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260261002
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  • 7
    Electronic Resource
    Electronic Resource
    Heat transfer from a buoyant smoke layer beneath a ceiling to a sprinkler spray. 2 - an experiment (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Fire and Materials 3 (1979), S. 34-38 
    Details
    ISSN: 0308-0501
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: An experiment is described which measures the heat transferred from a hot, flowing, buoyant gas layer in a mall, to a sprinkler spray. Interpolation techniques are described which allow for the large heat losses occurring within the apparatus (a large-scale model mall). The results are compared with predictions based on the method developed in Part 1 and found to be consistently 150% of the predicted heat transfer rates. Given the assumptions required by the predictive calculations this is thought to be reasonable degree of agreement. The largest heat transfer rate to the one sprinkler spray in this experiment was 157 kW ± 38 kW.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fam.810030107
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