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Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR (1997)

Huang, N. ; Angeles, E. R. ; Domingo, J. ; [et al.]

Springer

Theoretical and applied genetics 95 (1997), S. 313-320

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ISSN: 1432-2242

Keywords: Key words Rice ; Bacterial blight ; Resistance genes ; Pyramiding ; MAS

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract DNA marker-assisted selection was used to pyramid four bacterial blight resistance genes, Xa-4, xa-5, xa-13 and Xa-21. Breeding lines with two, three and four resistance genes were developed and tested for resistance to the bacterial blight pathogen (Xanthomonas oryzae pv. oryzae). The pyramid lines showed a wider spectrum and a higher level of resistance than lines with only a single gene. To speed up the gene pyramiding process and to facilitate future marker-aided selection, we developed PCR markers for the two recessive genes, xa-5 and xa-13, and used these to survey a range of rice germplasm. The results of the germplasm survey will be useful for the selection of parents in breeding programs aimed at transferring these bacterial blight resistance genes from one varietal background to another.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001220050565

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Quantitative and qualitative changes of plasma membrane glycoproteins in the early period of liver regeneration (1984)

Enrich, C. ; Bachs, O. ; Rius, E. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Cell Biochemistry and Function 2 (1984), S. 269-275

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ISSN: 0263-6484

Keywords: Liver ; plasma membrane ; glycoproteins ; sialic acid ; liver regeneration ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Alterations of cell surface glycoconjugates have been observed in many developing systems and may be important in the physiological control of growth and differentiation. Liver regeneration after partial hepatectomy is a suitable model in which to study the regulatory mechanisms of cell proliferation in vivo. We have isolated the sinusoidal plasma membrane of hepatocytes at different times after partial hepatectomy. The sialic acid content and the SDS-polyacrylamide gel electrophoresis pattern of glycoproteins were determined. A decrease of periodic acid-Schiff-profiles, a change in the binding capacities of 125I-concanavalin A, a reduction of the sialic acid content and the appearance and disappearance of specific components have been observed during the pre-replicative phase of liver regeneration. These findings during this early period are consistent with the active involvement of the plasma membrane glycoproteins in the transition of cells to the proliferative state.

Additional Material: 5 Ill.