ISSN:
0006-3525
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
An explicit relation between the percentage (G + C)-content XGC, and the melting temperature (Tm) of a natural DNA, is derived froma a statistical mechanics calculation with due consideration given to the base pair bonding and stacking interactions. The well-known Marmur-Doty empirical formula, linearly relating these two quantities, can now be understood in terms of the fundamental processes. We also propose a simpler experimental procedure for the determination of XGC. It is similar to the melting temperature method, but we take only one reading at a prescribed temperature of any natural DNA in addition to the two normalization readings. It should be more sensitive for DNAs having XGC ranging from 35 to 65%.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bip.1977.360160605
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