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  • 1
    Electronic Resource
    Electronic Resource
    Localization of an SLG protein expressed under the regulation of a tapetum-specific promoter in anthers of transgenic Brassica napus (1998)
    Springer
    Sexual plant reproduction 11 (1998), S. 245-250 
    Details
    ISSN: 1432-2145
    Keywords: Key words Anther ; Self-incompatibility ; S-locus glycoprotein ; Tapetum-specific promoter ; Transgenic Brassica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  S-locus glycoprotein (SLG) is known to be one of the proteins related to self-incompatibility in Brassica, and its transcripts are detected in anthers as well as stigmas. However, an SLG protein has not been detected in anthers so far. Because of sporophytic control of the self-incompatibility (SI) phenotype of pollen, an SLG gene is expected to be expressed in the sporophytic tissue of anthers, i.e., the tapetum. Overexpression of an SLG gene in the tapetum would enable us to predict the localization and function of an SLG protein in anthers. In this study, an SLG gene of self-incompatible B. campestris under the control of a tapetum-specific promoter was introduced into self-compatible B. napus. Immunoblot analysis using anti-SLG antiserum detected the exogenous SLG protein in the immature anthers, but not in the mature anthers. Immunoelectron microscopy showed the SLG protein to be localized in the tapetum and in the exine cell wall layer at the stage when the tapetum was degenerating. This result indicates the possible movement of the SLG protein from the tapetum to the pollen surface. A pollination test indicated that the pollen of the transgenic B. napus did not gain the SI phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050149
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  • 2
    Electronic Resource
    Electronic Resource
    Tapetum-specific expression of the Osg6B promoter-β-glucuronidase gene in transgenic rice (1997)
    Springer
    Plant cell reports 16 (1997), S. 363-367 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAgrobacterium ; Tapetum-specific promoter ; Transformation ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The promoter of an anther tapetum-specific gene, Osg6B, was fused to a β-glucuronidase (GUS) gene and introduced into rice by Agrobacterium-mediated gene transfer. Fluorometric and histochemical GUS assay showed that GUS was expressed exclusively within the tapetum of anthers from the uninucleate microspore stage (7 days before anthesis) to the tricellular pollen stage (3 days before anthesis). This is the first demonstration of an anther-specific promoter directing tapetum-specific expression in rice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01146774
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Tapetum-specific expression of theOsg6B promoter-β-glucuronidase gene in transgenic rice (1997)
    Springer
    Plant cell reports 16 (1997), S. 363-367 
    Details
    ISSN: 1432-203X
    Keywords: Agrobacterium ; Tapetum-specific promoter ; Transformation ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The promoter of an anther tapetum-specific gene,Osg6B, was fused to aβ-glucuronidase (GUS) gene and introduced into rice byAgrobacterium-mediated gene transfer. Fluorometric and histochemical GUS assay showed that GUS was expressed exclusively within the tapetum of anthers from the uninucleate microspore stage (7 days before anthesis) to the tricellular pollen stage (3 days before anthesis). This is the first demonstration of an anther-specific promoter directing tapetum-specific expression in rice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01146774
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Thermal decomposition of 3-chloro-3-trichloromethyldiazirine in solution (1972)
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 4 (1972), S. 229-233 
    Details
    ISSN: 0538-8066
    Keywords: Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The thermal decomposition of 3-chloro-3-trichloromethyldizairine in carbon tetrachloride and iso-octane has been investigated over the temperature range 75-115°C. The products, tetrachloroethylene and nitrogen, are formed quantitatively by a first-order reaction which is probably unimolecular: The results yielded the following Arrhenius equations: \documentclass{article}\pagestyle{empty}\begin{document}$$\begin{array}{l} k({\rm CCl}_{\rm 4} {\rm)} = {\rm 10}^{{\rm 13}{\rm .8} \pm {\rm 0}{\rm .2}} \exp (- 29,200 \pm 200/RT)\sec ^{ - 1} \\ k(iso - {\rm octane)} = {\rm 10}^{{\rm 13}{\rm .8} \pm {\rm 0}{\rm .2}} \exp (- 29,000 \pm 150/RT)\sec ^{ - 1} \\ \end{array}$$\end{document}.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/kin.550040209
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    Paper (German National Licenses)
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