ISSN:
1617-4623
Keywords:
Rice
;
ATPase subunit 6
;
Cytoplasmic male-sterility
;
Chimeric gene
;
Mitochondrial DNA
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Three ATPase subunit 6 (atp6) genes of rice mitochondria were isolated, one from normal and two from cms-Bo male-sterile cytoplasms, in order to determine whether the extra atp6 gene in cms-Bo rice plays a role in cytoplasmic male-sterility (CMS). The nucleotide sequences of all three genes were determined and analysis showed a chimeric atp6 gene (urf-rmc) as well as a normal atp6 gene in cms-Bo cytoplasm, but only the normal atp6 gene in normal cytoplasm. The urf-rmc gene is completely homologous to the normal atp6 gene from at least position − 426 in the 5′ flanking region to position + 511 downstream from the initiation codon ATG; however, the following downstream sequence shows no homology with the normal rice atp6 gene, or any other reported sequence. Introduction of the restorer of fertility gene altered transcription of the urf-rmc gene but not the atp6 gene, indicating participation of the chimeric gene in the expression of CMS. Southern blot analysis showed that the urf-rmc gene was generated by an intramolecular recombination event in mitochondrial DNA, and the homologous recombination point between the atp6 gene and the opposite ancestral sequence was identified as 5′-TTCCCTC-3′.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00259445
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