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  • 1
    Electronic Resource
    Electronic Resource
    Large scale purification and immunological characterization of human placental nerve growth factor (1990)
    Springer
    Neurochemical research 15 (1990), S. 1197-1202 
    Details
    ISSN: 1573-6903
    Keywords: Nerve growth factor ; human placenta ; immunological cross-reactivity ; monoclonal-polyclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Nerve growth factor (NGF) is a protein which plays a critical role in the development and survival of not only peripheral neurons, but possibly also cholinergic brain neurons. The present study describes a procedure for large scale isolation of human NGF of placental origin, and its immunological characterization. A protein species of approximately 26 kDa was obtained, which crossreared characterization. A protein species of approximately 26 kDa was obtained, which crossreacted with antibodies to mouse NGF. Polyclonal and monoclonal anti-mouse NGF antibodies appeared to recognize different bands within this human NGF preparation. Although these polyclonal antibodies recognized both the dimeric and monomeric forms of mouse NGF, the monoclonal antibody recognized only a band corresponding to the dimeric form of mouse NGF.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01208580
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Nerve growth factor antibodies recognize neurotrophin-3 (1993)
    Springer
    Neurochemical research 18 (1993), S. 705-709 
    Details
    ISSN: 1573-6903
    Keywords: Nerve growth factor ; brain-derived neurotrophic factor ; neurotrophin-3 ; antibody ; crossreactivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The immunological properties of the neurotrophins NGF, BDNF, and NT-3 were compared using polyclonal and monoclonal antibodies against the β subunit of mouse NGF. Affinity-purified anti-NGF IgG consistently recognized NGF and NT-3 on Western blots, and inhibited the trophic activity of NGF and NT-3 but not BDNF. In contrast, anti-NGF monoclonal antibodies did not block the trophic activity of either NT-3 or BDNF. These results are consistent with the greater structural overlap between NGF and NT-3 than between NGF and BDNF.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00966785
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Structure-function studies of human ciliary neurotrophic factor (1994)
    Springer
    Neurochemical research 19 (1994), S. 223-227 
    Details
    ISSN: 1573-6903
    Keywords: Ciliary neurotrophic factor ; mutagenesis ; secondeary protein structure ; biological activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Ciliary neurotrophic factor (CNTF) is a polypeptide that promotes the survival and/or differentiation of a number of neural cell types. Here we present a structural and functional analysis of the human CNTF molecule. Variant proteins were synthesized byEscherichia coli trnsformmed with mutant cDNA constructs, and purified by SDS-polyacrylamide gel electrophoresis and reverse phase high pressure liquid chromatography. Most variant CNTF proteins lacked neurotrophic activity, but two N-and C-terminal deletions (Δ2–14 and Δ173–200, respectively) actually displayed a several-fold increase in specific activity. Loss of biological activity was accompanied by changes in the alphahelical nature of CNTF as measured by circular dichroism. These data strengthen the proposed similarity between CNTF and the family of hematopoietic cytokines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00966820
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    Paper (German National Licenses)
    Fulltext
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