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  • Cloning vector construction  (1)
  • E. coli  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Construction of a new vector for the expression of foreign genes inZymomonas mobilis (1986)
    Springer
    Journal of industrial microbiology and biotechnology 1 (1986), S. 9-15 
    Details
    ISSN: 1476-5535
    Keywords: Cloning vector construction ; Expression ; Zymomonas mobilis ; Isolation of promoters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Broad host range plasmids have previously been shown to be suitable as vectors to introduce antibiotic resistance genes intoZ. mobilis. However, attempts to use these vectors to carry other genes with enteric promoters and controlling elements have resulted in limited success due to poor expression. Thus we have constructed a promoter cloning vector in a modified pBR327 and used this vector to isolated 12 promoters fromZ. mobilis which express various levels of β-galactosidase inEscherichia coli. Four of these were then subcloned into pCVD 305 for introduction intoZ. mobilis. All expressed β-galactosidase inZ. mobilis with activities of 100 to 1800 Miller units. One of these retained aBamHl site into which new genes can be readily inserted immediately downstream from theZ. mobilis promoter. Genetic traits carried by pCVD 305 were initially unstable but spontaneous variants were produced during sub-culture in which the plasmid was resistant to curing at elevated temperature. One of these variants was examined in some detail. The increased stability of this variant appears to result from an alteration in the plasmid rather than a chromosomal mutation or from chromosomal integration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569411
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Ethanol production from hemicellulose hydrolysates of agricultural residues using genetically engineeredEscherichia coli strain KO11 (1996)
    Springer
    Journal of industrial microbiology and biotechnology 16 (1996), S. 42-47 
    Details
    ISSN: 1476-5535
    Keywords: ethanol ; E. coli ; biomass ; lignocellulose ; pentose ; hemicellulose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Hemicellulose hydrolysates of the agricultural residues bagasse, corn stover, and corn hulls plus fibers were readily fermented to ethanol by recombinantEscherichia coli strain KO11. Corn steep liquor and crude yeast autolysate served as excellent nutrients. Fermentations were substantially complete within 48 h, often achieving over 40 g ethanol L−1. Ethanol yields ranged from 86% to over 100% of the maximum theoretical yield (0.51 g ethanol g sugar−1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569920
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    Paper (German National Licenses)
    Fulltext
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