ISSN:
1432-0584
Keywords:
Leukocytes
;
Colony forming units
;
Assay
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The use of peripheral blood feeder layers as a source of stimulus for colony formation by human granulocytic progenitor cells in semi-solid agar cell cultures was examined. Comparison with various conditioned media demonstrated that cultures stimulated by peripheral blood feeder layers produced the greatest number and largest colonies. The cell concentration in the feeder layers was more important than the total cell number. Feeder layer plates containing 2 × 105 cells at a concentration of 1 × 106 cells/ml proved to be just as potent as the conventional feeder layer plates containing 1 × 106 cells/ml in 1 ml. Thus feeder layer plates can be more economical in terms of cell numbers than has previously been reported. By using a known panel of donors for the leukocytes, the number of sub-optimal batches of feeder layers was reduced. Addition of 1 μM adenosine 3′ : 5′-cyclic-monophosphate or 10 μg/ml Li2CO3 to poor feeder layers enhanced their colony-stimulating ability.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00320694
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