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Digitale Medien

Rapid detection of hemoglobin variants by mutagenically separate polymerase chain reaction (MS-PCR) (1995)

Chang, J. G. ; Chang, C. P. ; Lu, C. M. ; [weitere]

Springer

Annals of hematology 71 (1995), S. 97-100

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ISSN: 1432-0584

Schlagwort(e): Hemoglobin variant ; MS-PCR ; Allele-specific primer

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract The detection of molecular defects of hemoglobin variants using mutagenically separated polymerase chain reaction (MS-PCR) was applied in this study. Using different lengths of allele-specific mutagenic primers, normal and mutant alleles of hemoglobin genes were amplified in the same reaction tube. Subsequent gel electrophoresis showed at least one of the two allelic products at the same loci or at least two of the several allelic products at different loci. We employed MS-PCR to test the following hemoglobin variants: Hb Constant Spring (Hb CS), Hb E, Hb G-Taichung, Hb J-Meinung, and Hb Kaohsiung. The results were the same as those obtained by amplified created reaction sites (ACRS) or direct sequencing. We conclude that the MS-PCR provides a rapid and simple alternative to other techniques for mutation detection in hemoglobin variants. Moreover, the principle can be extended to other genetic diseases.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01699253

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Digitale Medien

High-performance liquid chromatography of carbohydrates in enzymic hydrolysate of waste paper (1992)

Cheng, C. ; Chen, J. -T. ; Huang, H. -R.

Springer

Chromatographia 34 (1992), S. 534-536

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ISSN: 1612-1112

Schlagwort(e): Column liquid chromatography ; Carbohydrates ; Enzymic hydrolysate ; Waste paper

Quelle: Springer Online Journal Archives 1860-2000

Thema: Chemie und Pharmazie

Notizen: Summary Waste paper was pretreated with 5M sulfuric acid at 28°C to give a gel-like solution which was then hydrolyzed with cellulase, purified fromTrichoderma viride, to form carbohydrates. The determination of the carbohydrates was accomplished using an aminopropylbonded silica HPLC column and an RI detector. The detection limits were 20 ppm; xylose, glucose, and cellobiose were the major components. Salts from the buffer solution may affect the activity of cellulase. The analysis of carbohydrates can be performed under acid conditions. HPLC analysis of carbohydrates from enzymatic hydrolysis is reliable and successful.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF02290248

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