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  • 1
    Electronic Resource
    Electronic Resource
    An efficient Agrobacterium tumefaciens-mediated transformation and regeneration system for cotyledons of spinach (Spinacia oleracea L.) (1999)
    Springer
    Plant cell reports 18 (1999), S. 640-645 
    Details
    ISSN: 1432-203X
    Keywords: Key words Spinach (Spinacia oleracea L.) ; Regeneration ; Green-fluorescent protein ; Transformation ; Cotyledon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An efficient transformation and regeneration system was established for the production of transgenic spinach (Spinacia oleracea L.) plants. Cotyledon explants were infected with Agrobacterium tumefaciens strain LBA4404 carrying the selectable marker gene, neomycin phosphotransferase II (nptII), and the reporter gene smgfp, encoding soluble-modified green-fluorescent protein, driven by the cauliflower mosaic virus 35S promoter. The infected explants were cultured on Murashige and Skoog medium, containing 1 mg/l benzyladenine and 0.4 mg/l naphthaleneacetic acid. Shoots were regenerated on selection medium containing 50 mg/l kanamycin. Regenerated kanamycin-resistant shoots were rooted on medium containing 1 mg/l indolebutyric acid and subsequently grown in soil in the greenhouse. Southern blot analysis indicated that the smgfp gene had been integrated into the spinach genome. Northern and Western blots showed that the smgfp gene was expressed in progeny plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050635
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Complexity measure and complexity rate information based detection of ventricular tachycardia and fibrillation (2000)
    Springer
    Medical & biological engineering & computing 38 (2000), S. 553-557 
    Details
    ISSN: 1741-0444
    Keywords: Ventricular tachycardia ; Ventricular fibrillation ; Complexity measure ; Complexity rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract On the basis of non-linear dynamics, the paper uses a Lempel-Ziv complexity measure and presents a new definition of the information complexity rate: cc(n). Using such a definition, relative properties are obtained to help identify chaotic process accurately. Applying complexity analysis to abnormal ECGs recorded from patients with an implantable cardioverter defibrillator, the reasonableness of this information complexity and complexity rate approach are confirmed by means of biological experiments and computer simulations. Finally, objective analysis and explanations of the mechanisms of VT and VF are reported. The results indicate that, with the help of the complexity measure and complexity rate, recognition of ventricular tachycardia (VT) and ventricular fibrillation (VF) signals can be achieved with accuracy up to 100% (VT: 100%; VF: 98.7%).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02345752
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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