ISSN:
1432-203X
Keywords:
Key words Spinach (Spinacia oleracea L.)
;
Regeneration
;
Green-fluorescent protein
;
Transformation
;
Cotyledon
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract An efficient transformation and regeneration system was established for the production of transgenic spinach (Spinacia oleracea L.) plants. Cotyledon explants were infected with Agrobacterium tumefaciens strain LBA4404 carrying the selectable marker gene, neomycin phosphotransferase II (nptII), and the reporter gene smgfp, encoding soluble-modified green-fluorescent protein, driven by the cauliflower mosaic virus 35S promoter. The infected explants were cultured on Murashige and Skoog medium, containing 1 mg/l benzyladenine and 0.4 mg/l naphthaleneacetic acid. Shoots were regenerated on selection medium containing 50 mg/l kanamycin. Regenerated kanamycin-resistant shoots were rooted on medium containing 1 mg/l indolebutyric acid and subsequently grown in soil in the greenhouse. Southern blot analysis indicated that the smgfp gene had been integrated into the spinach genome. Northern and Western blots showed that the smgfp gene was expressed in progeny plants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050635
