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  • Computational Chemistry and Molecular Modeling  (6)
  • Hematoxylin stainable protein  (3)
  • Prognosis  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    FEBS Letters 267 (1990), S. 261-264 
    ISSN: 0014-5793
    Keywords: Cystatin-α ; Cysteine proteinase inhibitor ; Hematoxylin stainable protein ; Keratohyalin granule
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0014-5793
    Keywords: Cystatin α. ; Hematoxylin stainable protein ; Phosphorylated cysteine proteinase inhibitor
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-069X
    Keywords: Hematoxylin stainable protein ; Keratohyalin granules ; Membrane lining protein ; Stratum corneum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A monoclonal antibody (Ted-R-1) to the keratohyalin granules (KHGs) of the newborn rat epidermis was developed by immunizing mice with a pH 4.7 hematoxylin-stainable fraction of the extract in a 50 mM Tris-HCl buffer, pH 7.3, containing 10 μ/ml phenylmethylsulfonyl fluoride (PMSF) from the newborn rat whole epidermis. Using an indirect immunofluorescence technique, Ted-R-1 was located in two places: (a) the KHGs and (b) the cell membrane region of the stratum corneum. Under immunoelectron microscopy, the antigenic materials were located at the periphery of the KHGs. Immunoblotting analyses of the antigenic pH 4.7 fraction, the Tris-HCl extract from the stratum corneum, and the Tris-8M urea extract from freeze-dried whole skin demonstrated that there were 55 kd, 43 kd, and 78 kd polypeptides, respectively, with which Ted-R-1 reacts. No possitive spot was seen in the dermal extract. The major amino acids of the 55 kd protein were glycine (24.4%), serine (17.3%), glutamic acid (14.1%), and alanine (7.7%), while the histidine residue was only 3.1%. On the basis of its amino acid composition, the 55 kd protein is the third component of KHGs — in addition to histidine-rich and cystinerich proteins.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1459
    Keywords: Guillain-Barré syndrome ; Nerve conduction velocity ; Prognosis ; Motor paralysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Klinisch-elektrophysiologische Untersuchungen wurden bei 39 Patienten mit Guillain-Barré Syndrom durchgeführt. Es ging um die Frage, welche Elemente in der akuten Phase eine prognostische Beurteilung zulassen. Bei 16 der Patienten, was 52% des Krankengutes entspricht, wurden residuelle Symptome wie motorische Schwäche, fehlende Patellarsehnenreflexe und so weiter nachgewiesen. Das Zurückbleiben von residuellen Symptomen korrelierte mit dem Alter bei Krankheitsbeginn, das Ausmaß der Tetraparese und das Ausmaß der Sensibilitätsstörungen in der akuten Phase. Acht der zehn Patienten, die eine Verminderung der motorischen Nervenleitgeschwindigkeit in einer frühen Krankheitsphase aufwiesen, zeigten später nennenswerte Restsymptome. Tendenzmäßig schienen Restsymptome häufiger bei jenen Patienten zu sein, welche eine Verlangsamung der Leitgeschwindigkeit gemischter Nerven und eine verlängerte Latenz der H-Welle sowie der Residuallatenz aufwiesen. Elektroneurographische Untersuchungen, im besonderen die Bestimmung der motorischen Leitgeschwindigkeit, erlauben zuverlässige Rückschlüsse auf die Prognose dieses Krankheitsbildes.
    Notes: Summary Clinical and electrophysiological studies were carried out on 39 patients with the Guillain-Barré syndrome to evaluate which elements were of prognostic value during the acute phase. Residual clinical signs such as motor weakness and absent patellar tendon reflexes were found in 16 (52%) of those patients who had had a preceding illness. Persistence of deficit was significantly correlated to age at onset, the degree of quadriparesis and loss of deep sensation in the acute phase. Of the 10 patients who showed a reduction in motor nerve conduction velocity (MCV) in the early stage, 8 (80%) revealed significantly residual clinical symptoms at follow-up. There was a tendency for the incidence of residual signs to be more common in the patients with slowing of mixed nerve conduction velocity, and prolonged latency of H-wave and the residual latency. Nerve conduction studies, especially measurement of MCV, were of value as a reliable prognostic indicator in this syndrome.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    European archives of oto-rhino-laryngology and head & neck 255 (1998), S. 311-314 
    ISSN: 1434-4726
    Keywords: Key words Parotid tumors ; Immunohistochemistry ; Proliferating cell nuclear antigen ; Prognosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We evaluated the prognostic value of immunostaining proliferating cell nuclear antigen (PCNA) by using a monoclonal antibody (PC10) in patients with parotid tumors. Twenty-seven cases were studied. Immunohistochemical studies were carried out on paraffin-embedded tissues from the patients, and the PCNA index was calculated as the percentage of positively staining tumor cells. The PCNA index ranged from 0.1 to 65.3%. We divided the 27 lesions into three groups histologically: group A with benign pleomorphic tumors (11 cases), group B with low-grade malignant tumors (5 cases), and group C with high-grade malignant tumors (11 cases). The mean PCNA index was 0.7% in group A, 2.0% in group B, and 23.1% in group C. The clinical data revealed a significantly higher local tumor recurrence and mortality rate in group C than in groups A and B. We conclude that PCNA may be used as an important indicator for determining clinical prognosis in parotid tumors.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    International journal of clinical oncology 5 (2000), S. 164-170 
    ISSN: 1437-7772
    Keywords: Key words P-glycoprotein ; Osteosarcoma ; Soft-tissue sarcoma ; Prognosis ; Immunohistochemistry ; RT-PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background. The purpose of this study was to investigate the correlation between P-glycoprotein status and outcome in adult patients with high-grade osteosarcomas and soft-tissue sarcomas. Methods. P-glycoprotein status was determined im-munohistochemically in specimens from 28 patients with osteosarcoma and 34 patients with soft-tissue sarcoma. The polyclonal antibody mdr(Ab-1) was used for either decalcified or undecalcified tissue samples which were formalin-fixed and paraffin-embedded. The expression of P-glycoprotein mRNA was also determined by the polymerase chain reaction in 23 fresh sarcoma specimens. P-glycoprotein status was analyzed in relation to the duration of event-free survival. Results. Positivity for P-glycoprotein was found in 29% of the osteosarcomas and 34% of the soft-tissue sarcomas. Consistent results were obtained at both the immunohistochemical and reverse transcriptase-polymerase chain reaction (RT-PCR) levels in 19 of 23 sarcomas (83%). In patients with osteosarcoma, the presence of increased levels of P-glycoprotein was significantly associated with a decreased probability of event-free survival after diagnosis (P = 0.022). In contrast, in patients with soft-tissue sarcoma there was no correlation between the level of P-glycoprotein and prognosis. Conclusions. In patients with high-grade osteosarcomas, the presence of increased levels of P-glycoprotein detected by polyclonal antibody mdr(Ab-1) was associated with a significantly increased risk of adverse events. This association was not found in patients with soft-tissue sarcomas.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 53 (1995), S. 457-466 
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The perturbation expansion coefficients for the ground-state energy of the half-filled one-dimensional Hubbard model with N = 4ν + 2, (ν = 1, 2,…) sites and satisfying cyclic boundary conditions were calculated in the Hückel limit (U/β → 0), where β designates the one-electron hopping or resonance integral, and U, the two-electron on-site Coulomb integral. This was achieved by solving - order by order - the Lieb-Wu equations, a system of transcendental equations that determines the set of quasi-momenta {ki} and spin variables {τα} for this model. The exact values for these quantities were found for the N = 6 member ring up to the 20th order in terms of the coupling constant B = U/2β, as well as numerically for 10 ≤ N ≤ 50, and the N = 6 Lieb-Wu system was reduced to a system of sextic algebraic equations. These results are compared with those of the infinite system derived analytically by Misurkin and Ovchinnikov [Teor. Mat. Fiz. 11, 127 (1972)]. It is further shown how the results of this article can be used for the interpolation by the root of a polynomial. It is demonstrated that a polynomial of relatively small degree provides a very good approximation for the energy in the intermediate coupling region. © 1995 John Wiley & Sons, Inc.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 26 (1984), S. 349-371 
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The finite-order many-body perturbation theory using the localized Wannier orbital basis is applied to the problem of bond length alternation in the Pariser-Parr-Pople model of cyclic polyenes CN HN, N = 4v + 2, which may be regarded as a simplified model of polyacetylene. Both the Møller-Plesset and the Epstein-Nesbet-type partitionings of the model Hamiltonian are employed. The localized orbital basis enables an efficient truncation of the perturbation theory summations over the intermediate states as well as an elimination of energetically unimportant diagrams, thus enabling one to obtain the fourth-order Møller-Plesset-perturbation energies with a relatively small computational effort even for large polyenes. The results obtained with the second-, third-, and fourth-order Møller-Plesset and with the third-order Epstein-Nesbet perturbation theories yield very similar bond length distortions (about 0.05 Å) and stabilization energies per site (about 0.04 eV) as obtained earlier with the RHF, one-parameter AMO, and delocalized orbital perturbation theories. The effects of truncation and diagram elimination in the fourth-order Møller-Plesset perturbation theory and the abnormal behavior of the second-order Epstein-Nesbet perturbation theory results in the localized Wannier basis near the instability threshold of the RHF solutions are discussed.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 28 (1985), S. 459-479 
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The role of electron correlation effects on the bond-length alternation in linear metalliclike systems, as modeled by cyclic polyenes CNHN, N = 2n = 4v + 2, v = 1,2,…, is examined using the coupled cluster approach in the localized Wannier basis formalism. A recently developed approximate coupled pair approach which accounts for connected quadruply excited clusters is employed together with various truncation schemes for the localized doubly-excited cluster components. It is found that for the physical value of the coupling constant, the electron correlation has only a very slight effect on the potential energy curves, yielding almost the same values for both the magnitude of the bond-length alternation and for the stabilization energy relative to the symmetric equidistant structures as the restricted Hartree-Fock theory. This is in contrast to a strongly correlated region where the correlation effects stabilize the undistorted non-alternating structures. Different mechanisms of the bond-length alternation or Peierl's distortion as implied by a simple Hückel Hamiltonian and by the Pariser-Parr-Pople Hamiltonian models are also pointed out.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 26 (1984), S. 237-244 
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Problems which arise in the application of closed-shell coupled-cluster approaches to quasidegenerate or almost degenerate situations are discussed and the basic classification of quasidegeneracy types is outlined. Recent coupled-cluster results obtained for the cyclic polyene model, particularly in the strongly correlated limit, are briefly discussed and the unexpected features of approximate and localized coupled-pair approaches are pointed out.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
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